| The research was established on the foundation of the nature that kidney yang asthenia syndrome exist the functional disorder of HPA axis.On the base of the pharmacodynamic research in early days,to observe that dismantled shenqi pill groups how to influence the HPA axis of kidney yang asthenia syndrome rats.And detect the content of IL-1β,IL-2,IFN-γ,TNF-αin lood plasm and 5-HT,DA in hypothalamus of rats,then detect the content of cinnamaldehyde,paeonol,aconitine in each shenqi pill decotion.Try to explain the pharmacodynamic mechanism and material foundation that compatibility of shenqi pill "reinforcing yang from yin" to treat kidney yang asthenia syndrome.To dismantle the whole shenqi pill into invigorating yang groups,invigorating yin groups and invigorating yin-yang groups.The first invigorating yang group is composed with aconite root and ramulus cinnamomi; the second invigorating yang group is composed with aconite root,ramuhs cinnamomi,hoelen,alisma rhizoma,moutan cortex;The first invigorating yin group is composed with rehmannia,asiatic cornelian cherry fruit,dioscorea root;The second invigorating yin group is composed with rehmannia,asiatic cornelian cherry fruit,dioscorea root,hoelen,alisma rhizoma,moutan cortex; The first invigorating yin-yang group is composed with aconite root,ramulus cinnamomi,rehmannia,asiatic cornelian cherry fruit,dioscorea root;The second invigorating yin-yang group is composed with with aconite root,ramulus cinnamomi,rehmannia,asiatic cornelian cherry fruit,dioscorea root,hoelen,alisma rhizoma and moutan cortex that is the whole shenqi pill group.To divide the SD male rats into normal group,model group and dismantled shenqi pill groups at random.Inject hydrocortisone to the rest rats at the dose of 25mg/kg/d for 12 day,except the normal group.And began to intragastric administration at the same time,to supply the normal group and model group with liquor natrii chloridi isotonicus in the same volume.And it last 20 days.Then to anesthetize the rats,get blood from the heart of rats at 21th day.To separated the blood and detect the content of IL-1β,IL-2,IFN-γ,TNF-αin blood plasma by the method of ELISA.Obtain hypothalamus,weigh it, add 5%perchloric acid,homogenate,centrifugalization and get supernate fluid, detect the content of DA,5-HT in hypothalamus by the method of high performance liquid chromatogram at last.On the basis of above-mentioned shenqi pill dismantled groups,to detecte the content of cinnamaldehyde,paeonol,aconitine in the decoction of groups that contain the ramulus cinnamomi,moutan cortex and aconite root.Then analysis the influence of different compatibilities,to approach the material foundation of the compatibility that shenqi pill "reinforcing yang from yin" to treat kidney-yang asthenia syndrome.The results of assay indicated that the content of IL-1β,IL-2 in blood plasm of model group rats decreased.After intragastric administration,the content of IL-1β,IL-2 in blood plasm increased of dismantled shenqi pill groups with the comparison of normal group.Among the results,the content of second invigorating yin-yang group is higher than second invigorating yang group,the content of first invigorating yin-yang groupis higher than first invigorating yang group,the content of IL-1βof first invigorating yin-yang group is higher than first invigorating yin group,all these posses statistics meaning.Compared with normal group,the content of IFN-γ,TNF-αin normal group decreased.After intragastric administration,the content of IFN-γ,TNF-αin blood plasm increased of dismantled shenqi pill groups at different level,but there is no obviously different among groups.Compared with normal group,The content of DA,5-HT increased in hypothalamus of normal group rats.After intragastric administration,the content of DA,5-HT in hypothalamus decreased of dismantled shenqi pill groups.Invigorating yin-yang groups cut down to the lowest,and invigorating yang groups decreased unobviously.Among the results,the content of first invigorating yin-yang group is lower than first invigorating yang group,the content of second invigorating yin-yang group is lower than second invigorating yang group.And all these posses statistics meaning.To detect the content of cinnamaldehyde in decotion of each dismantled shenqi pill group that contains ramulus cinnamomi.The results indicate that the content of cinnamaldehyde in decotion of second invigorating yin-yang group is higher than second invigorating yang group.The content of first invigorating yin-yang group is higher than first invigorating yang group,all these posses statistics meaning.The content of cinnamaldehyde in decotion of second invigorating yang group is higher than first invigorating yang group. The content of second invigorating yin-yang group is higher than first invigorating yin-yang group,and these also posses statistics meaning,lt means that the content of cinnamaldehyde in decotion of each group that contains hoelen,alisma rhizoma,moutan cortex is higher than the same group that discontain hoelen,alisma rhizoma,moutan cortex,lt exist not only in the pure invigorating yang groups but also in invigorating yin-yang groups.To detect the content of paeonol in decotion of each dismantled shenqi pill group that contain moutan cortex.The content of second invigorating yin group and second invigorating yin-yang group is higher than invigorating yang group,and it posses statistics meaning.Although the content of second invigorating yin group is higher than second invigorating yin-yang group,it has no statistics meaning.From the experimental result,we can infer that the increased content of IL-1β,IL-2 in blood plasm and the decreased content of DA,5-HT in hypothalamus are likely to be the mechanism that shenqi pill to treat the kidney-yang asthenia syndrome by the compatibility of "reinforcing yang from yin" Cinnamaldehyde and paeonol are likely to be the effective constituent of the compatibility of shenqi pill' "reinforcing yang from yin" of that shenqi pill to treat kidney yang asthenia syndrome.
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