Effect Of Traditional Chinese Drug On Fertilization And The Development Rate Of Murine Embryo In Vitro

Traditional Chinese drug is our country's endemic resource. Active component's combination in traditional Chinese drug have special effect on treating barrenness, abortion, tocolysis and promoting motility of sperm in animal breeding. And it also have the effect of Anti-lipid peroxidation, cleaning up free radical, promoting oocyte maturation, cell proliferation. Co-culture is a new cultural method in vitro.It can prolong the culture time of embryo in vitro.Accum efficiency of embryo producting in votro is low.The neck of restricting is the longevity of oocyte and abnor oocyte.The purpose of this study is to elevating the accum efficiency through approaching a safe method which can culture mouse endometrium epithelial cell,then approaching a traditional Chinese drug which is good for fertilization and embryo culture in vitro,and searching the right time of admoveaturing,at last estabilishing a reasonable sequential co-culture system.To studying the effect of right Traditional Chinese drug on fertilization and embryo culture in vitro.The first stage is to approaching a safe method which can culture mouse endometrium epithelial cell,and getting purity endometrium epithelial cell.The second stage is to approaching a traditional Chinese drug which is good for fertilization and embryo culture in vitro,and searching the right time of admoveaturing through observing the effect of three traditional Chinese drug admoveaturing on different time on fertilization and embryo culture in vitro.The last stage is to integrating the result before, and observing the effect of new sequential co-culture system on fertilization and embryo culture in vitro.The results indicate that:(1) The mouse endometrium epithelial cells could be isolated by using enzyme digestion,But the purity ,the vigor and the proliferation velocity of cell was influenced by the enzyme. At the same time, the purity ,the vigor and the proliferation velocity of cell was not influenced by using the method of turnover uterus tissue.This method was used for providing somatocyte to studying sequential co-culture.(2) The effect of 0.5mg/L ligustrazine and 4mg/L baicalin on fertilization and embryo culture in vitro was not significant(P>0.05),but they could delay the development of embryo after 2-cell stage. 0.1mg/L berberine could protect oocyte on the process of fertilization and make the rate of oocyte be significantly elevated(49.5% control group 7.5%).but it was bad for culturing of fertilized ovum (P<0.05).(3) The rate of 2-cell development arrest of embryo which cultured in co-culture system is lower than the control group(P<0.05).