Effects Of Static Magnetic Field On Cytosolic Free Calcium Of Rat Maxillofacial Skeletal Muscle Cells In Vitro

Objectives:To establish the model of culture SD rat maxillofacial skeletal muscle cells in vitro,and observe the biological effect of static magnetic field(SMF)by the NdFeB rare earth magnets used in orthodontics on cytosolic free calcium and calcium ion channel of rat maxillofacial skeletal muscle cells,discussing and approaching the likelihood mechanism of magnetic field biology effect.Methods:The maxillofacial skeletal muscle cells of neonatal 2～3 day age SD rat were isolated and cultured in vitro,and the cultured maxillofacial skeletal muscle cells of the third generation were treated with SMF for 1d,2d,3d,4d,5d.Observe and record the growth state of cutured cells under the inversion phase microscope;with colorimetry mensuration ATP enzyme activity;Fluo-3 loaded with maxillofacial skeletal muscle cells,and measure cytosolic free calcium separately by flow cytometry and laser confocal scanning microscope.Result:After the exposure of SMF,the cells grew well in the magnetic field and the biological compatible was well. Intracellular calcium ion concentration of cultured cells increased slightly compared with the control group,but with no significant statistical difference in the control group.The activity of intracellular Ca²⁺-ATPase was enhanced after 3-day exposure in SMF.Conclusion:No pathological damage is found in the maxillofacial skeletal muscle cells exposed in orthodontic magnets.The cutured cells grow well in the magnetic field,and the magnetic field can promote the activity of intracellular Ca²⁺-ATPase,and regulate cutured cells' proliferation.