Static Magnetic Fields Of Application On The Orthodontic Clinics And Researching Its Biological Safety In The Maxillofacial Skeletal Muscle Cells

Traditional orthodontic force came from a variety of mechanical force and muscle strengths. In 1978, Blechman and Smiley used permanent magnets to move cats’canines, and this magnetism conception was introduced into orthodontic fields. In the following 20 years, magnetic force has been applied to research and in clinics. At present, magnetic force, which is a new mechanical system, has been widely recognized. Through overseas research and years of domestic magnetic functional orthopedics, clinical and animal studies have found that the use of high energy permanent magnets have greatly increasd the treatment efficiency, particularly for the correction of dentofacial deformities has unique advantages. Permanent magnets that are small and can be easily embedded in the appliance, it is very convenient to patients while also generating enough power to obtain a better clinical effect. As the process of magnetic correction must coexist with the magnetic field, study of the magnetic field on the organization of the biological effects of oral and maxillofacial orthodontics has become an important research direction in the field.This study of the static magnetic fields through the establishment in vitro model of skeletal muscle cells, respectively 12h、36h、60h, observes the muscle cell morphological changes by the microscope after the static magnetic fields expose. For more in-depth study of intracellular calcium ion changes in the static magnetic fields, the following provides guidance. By the LSCM, intracellular calcium concentration in the skeletal muscle cells can be seen changing. The results show: With the increase of the magnetic field exposure time, intracellular calcium also increases the fluorescence intensity; at the same time, the calcium ion concentration maintains a directly proportional relationship with the magnetic field strength.The Ca²⁺-Mg²⁺-ATP enzyme is widely distributed in the body of the membrane enzyme system; it maintains the normal function of cells that plays an important role. PCR is used to detect Ca²⁺-Mg²⁺-ATP enzyme changes in skeletal muscle cells after static magnetic fields are exposed.IP₃R （inositol trisphosphate receptor） is a class of ligand （IP₃）-operated calcium release channels. IP₃R changes in the number and structure may affect the intracellular calcium signal amplitude and frequency. Western-blotting was used to detect IP₃R protein in membrane after expose in static magnetic field.This paper studies cell biology, electrophysiology and molecular biology of the static magnetic field on the maxillofacial skeletal muscle cells, it will provide a theoretical foundation for application of magnetic materials in the field of orthodontics.