The Effect Of IGF-1 On The Induced Differentiation Of Ectomesenchymal Cells Of SD Fetal Rat Mandibular Process To Osteoblast In Vitro

OBJECTIVE:The purpose of this study is to develop a culturing model for the rat undifferentiated ectomesenchymal cells;to observe the consequential changes of the ectomesenchymal cells cultured in monolayer with insulin-like growth factor-1(IGF-1)at the different phases,at the same time to investigate the process of IGF-1 on inducing the ectomesenchymal cells to differentiate to osteoblasts.METHODS:The rat ectomesenchymal cells were separated by trysin,The ectomesenchymal cells of SD fetal rat mandibular were cultured in vitro,therefor the incubated models were established.The third passages of mandibular process ectomesenchymal cells were cultured for 10 days in DMEM/F 12 containing 20 ng/ml IGF-1 with 50 ml/ L FBS.The morphology was observed by phase -contrast microscopy and HE coloration.Immunohistochemistry was used to identify the characteristics of cells.Alkaline phosphatase was tested.RESULTS:The cultured ectomesenchymal cells appeared to be fibroblast - like cells, Anti - Vimentin were positive by immunohistochemical stain,and Anti-Cytokeratin was negative.There were significant changes in the morphology of the cells after 10 days cultured in IGF-1.The cells changed from fibroblast - like to multilateral and cuboid.Anti - collogen type-â… was positive.Alkaline phosphatase stain was positive and Cells showed increased.Alkaline phosphatase activity. CONCLUSION:Ectomesenchymal cells can be differentiated to osteoblasts by insulin-like growth factor-1.The cells changed from fibroblast-like to multilateral and cuboid.The cells secreted Alkaline phosphatase and collogen type-â… ,There are the features of the osteoblast.