Effects Of IGF-1and FGF-2on Osteoblast Differentiation Of Periodontal Ligament Stem Cells

Objective:Effects of insulin-like growth factor-1(1GF-1) and fibroblast cell growth factor-2(FGF-2) on periodontal ligament stem cells (Periodontal ligament stem cells, PDLSCs) to differentiate into osteoblasts.Methods:Collagenase digestion of human periodontal ligament tissue to get periodontal ligament stem cells (PDLSCs). PDLSCs were conducted biological detection by the inverted microscope, HE staining, flow cytometry after in vitro identification, amplified. Osteoblast induction medium adding25ng/ml IGF-1and0.5nmol/L of FGF-2sustained induction of7and14days, than alkaline phosphatase staining, alkaline phosphatase activity detection, and alizarin red stainingand real-time quantitative PCR assay to the sign of osteoblast differentiation genes (of Runx2, Alp, col-1) expression.Results:IGF-1stimulation group, the ALP activity and calcified nodules was significantly higher than that of control, mRNA of Runx2, Alp, col-1were highly expressed; ALP activity and calcified nodules of the FGF-2stimulation group was higher than that of controls and Runx2, Alp, col-1mRNA expression levels higher than the control.Conclusions:IGF-1and FGF-2promote the vitro PDLSCs direction of differentiation to osteoblasts in varying degrees.