The Initialized Studies On Anticancer Active Constituents From Phellodendron Chinense Schneid And Croton Tiglium L. Growing In Guangxi

The search for leading compound with important biological activities from natural plants is one of the most important approaches in modern drug discovery. The results of the anticancer active constituents from P. chinense schneid and Croton tiglium L. which growing in Guangxi.have been reported.Part 1: The studies on anticancer active constituents from P. chinense schneid(PCS)PCS is a plant of Rutaceae phellodendron, wide spreadly distributes in Sichuan, Yunnan, Guizhou, Hubei, Guangxi, Shandong, Gansu, Zhejiang provinces. It has been used as astomachic for intestinal function control, as a febrifugal agent and an anti-inflammatory agent. Recently, renferences reported: PCS has inhibition to BGC823. Studies on the PCS maily including three parts. At first combed with the silica gel column and TLC technology, seven compounds were isolated from a MeOH extract of PCS, and their structures were determined by spectroscopic methods (IR, 1H-NMR, 13C-NMR, ESI-MS, EI-MS). They were Berberine (1),β-sitosterl (2), Stigmasterol (3), ferulic acid (4), 3,4-dihydroxy benzoic acid (5), Kaempferol-7-o-gluc (6), obaculactone (7). Compounds (5), (6) were firstly obtained from PCS. Compounds (7) was obtained as crystal at the first time. Secondly searched in the antcancer activity of this plant, it has been found that this plant has good inhibition to MGC803. Third, considering the effect of TCM is not only the organic compositions but also the inorganic compositions such as metal microelement. PCS were determined the content of metal microelement by ICP-AES.and the result indicates that Ca,Mn,Sr,Fe,Zn,Cu is rich. Those microelement may be had pharmacological activity.Part 2: The studies on antcancer active constituents from Croton tiglium L.Croton tiglium L. is a plant of euphorbiaceae croton. This plant is found widely in south of Zhejiang, Fujian, Jiangxi, Guangdong, Hannan, Guangxi, Yunnan provinces, and is famous as its poisonous. The seeds, barks and leaves are good pesticide agent. It had been used to cure malignant, arthritis and so on. Recently, It is that Croton tiglium L. had the effection of antcancer.activity. Studies on the Croton tiglium L. maily included three parts: Firstly, studies on the compounds and anticancer activity of this plant. We found that this plant had good inhibition to ten tumorous cells, and It may be those active compounds are diterpenoids esters. Secondly, the constituents of essential oil from the leaves of Croton tiglium L. were analyzed by GC-MS and were studied on the anticancer activity. Essential oil has good anticancer activity to HCT116.Lastly, the content of metal microelement in Croton tiglium L. were determined By ICP-AES. K, Cu, Fe, Zn, Ca, Mg, Mn, Al, Sr, Na were rich in Croton tiglium L.and those microelements had pharmacological activity in Croton tiglium L.To summarize, It has been studied on the chemical constituents and anticancer activity of PCS and Croton tiglium L. The chemical constituents and activity parts of those two plants are known. In this thesis, the methanol extracts of PCS and Croton tiglium L were chemically investigated...