The Analysis Of Expression Of Liver Hepcidin Gene Of SD Rats Osteoporosis Model In Dexamethasone

Hepcidin is a kind of rich-cysteine antibiotic polypeptide, in 2001, Park separated this polypeptide first time from human urine. This kind of antibiotic peptide can make a good effect to iron dynamic equilibrium, meanwhile it regulate absorption, utilization, storage, recycle of iron alternative pathway at liver and macrophage. And iron metabolism has strong associativity with osteoporosis. So we research the relation between hepcidin and osteoporosis, it wil not only abundance influencing factors of osteoporosis but also establish the new way to osteoporosis treatment.We establish osteoporosis models of hormone SD rats, in order to detecte the tendency of expression of liver hepcidin gene in the formation process of rats osteoporosis model and investigate associations between hepcidin and osteoporosis. The study is divided into three parts:Partâ… The analysis of bone mineral density and serum Index between osteoporosis models in dexamethasoneObjective: To establish osteoporosis models in dexamethasone(Dex) SD rats, in order to understand Dex'influence to bone metabolism. Exploring at different time points, the distinction of bone mineral density(BMD) and serologic between the Dex group and control group.Methods: Choosing 6 months pure line female rats to i.m Dex 0.1 mg/100 g twice a week, for 6 weeks, then the hormone model of SD rats was established. At different time points , we compare the change of serum calcium, phosphorus, alkaline phosphatase (ALP) and BMD between the model group and control group. Results: At every time BMD of rats in Dex group point is lower than control group(P<0.05 or P<0.01). Otherwise at different time points the ALP level is higher(P<0.01) The serum calcium of Dex group is lower(P<0.05), but phosphorus is higher(P<0.05).Conclusion: The Dex group and control have some distinctions in serology index and BMD at different time points. Meanwhile we found that the succeed time of Dex group should be 5 weeks or even more.Partâ…¡The appearance of bone histomorphology of osteoporosis models in dexamethasoneObjective: To establish osteoporosis models in dexamethasoned(Dex) SD rats. Exploring the appearance and distinction of bone histomorphology between the Dex group and control group.Methods: Choosing 6 months pure line female rats to i.m Dex 0.1 mg/100 g twice a week, for 6 weeks. And. So that the osteoporosis models of SD rats was established. We compared the appearance of bone tissue with light microscope and scanning electron microscope(SEM).Results: The bone histomorphology of rats in Dex group present typical osteoporosis state. The bone trabecula shows collapse and osteocollagenous fibers appears cavitas.Conclusion: The Dex group displayed similarity and specificity with light microscope and scanning electron microscope. Partâ…¢The analysis of expression of liver hepcidin gene of SD Rats osteoporosis model in dexamethasoneObject: We establish osteoporosis models of dexamethasone SD rats, in order to detecte the tendency of expression of liver hepcidin gene in the formation process of rats osteoporosis model and investigate associations between hepcidin and osteoporosis.Methods: 80 SD rats to divide into experiment group and control group, every group is 40. To i.m Dex 0.1mg/100g twice a week, so that the osteoporosis model in hormone was established after 6 weeks. To i.m the indetical ml saline with control group. Then we measured the expresion of the liver hepcidin gene with RT-PCR at the 14th,21st,24th,28th,31st,35th,38th,42nd Day.Results: To compared with control group, hepatic hepcidin gene expression of test group on different stage had obviously differences. From model made to 17th day, hepcidin mRNA expression was obviously increasing. From 21th day to 38th day, contents of hepcidin remained unchanged. After 38th day, it was also obviously rose.Conclusion: The high expression of liver hepcidin in model rats liver may have some relations with osteoporosis formation. Hepcidin may become a new diadynamic criteria in abnormal change of bone mineralization, and offer a new proof to the clinical research of osteoporosis.