| Objective: Bullous keratopathy is the advanced stage appearance of corneal endotheliocyte function decompensation. The long-term persistent dropsy of corneal epithelium and parenchyma leads to the formation of water vacuoles on and below corneal epithelium. Bullous keratopathy is not a independent disease, it's originated from many ophthalmocace. In recent years, following with the pervasion of caligo lentis extirpation and artificial lens implantation, especially the application of anterior chamber intraocular lens, there is a obviously adscendent tendency in the attack rate of bullous keratopathy. It not only influences eyesight gravely but also induces irritation such as violent ocular region ache, phengophobia and dacryorrhea when water vacuoles rupture. Penetrativity corneal transplant is the radical cure to the patients with simple keratopathy. But as to the bullous keratopathy forming from part of ocular trauma or multiple ocular operation such as serious oculo-protomerite demolish or be accompanied with secondarg glaucoma and so on, there is no desire to revive the eyesight of the sick eye, so penetrativity corneal transplant is no longer the optimal therapeutic regimen. Therefore, in clinically the treatment of this part of patients is troublesome and difficult. In this study, slow release for basic fibroblast growth factor (bFGF) micropellets was implanted to cornea propria in rabbit with bullous keratopathy to induce corneal neovascularization. The treatment of corneal neovascularization on bullous keratopathy in rabbit was observed and we look forward to providing experimental evidence for the treatment of bullous keratopathy. Methods: 30 male or female healthy adult New Zealand rabbits and the weight of rabbits were between 2kg and 2.5kg.The model of bullous keratopathy was built by perfusing anterior chamber with 0.05% benzalkonium bromide solution. And then implant slow release for bFGF micropellets (500ng/pill) or micropellets without bFGF to cornea propria in lagophthalmos with affection. Cornea center thickness, the degree of corneal edema and corneal neovascularization were measured to analyze the effect of the experiment.Results: After perfusion with BAKB, all corneas were dropsical obviously during 24 hours; corneal bullae appeared four days later; many corneal bullea were observed , nubecula was obvious, iridial texture was not phanerous and presenting typical manifestation of bullous keratopathy two weeks later. In experiment group, new vessels began to erupt from corneoscleral junction average after the implantation of slow release for bFGF micropellets to cornea propria for 4±0.81d.The new vessels extended to the direction of insert, grew fascicularisly and surround insert gradually .The circumsciption of new vessels was limited, vessels vegetation was most intensive after about 10 days and there were no marked changes several days later. And there was no marked changes or natural extinctive tendency until four weeks. Following with the formation of CNV, corneal edema lessened, turbidity decreased and the bubble presented absorbability changes. In blank and control groups, there were no obvious changes in keratopathy after micropellets were implanted and the cornea also presented the condition of bullae.Conclusion: Perfusing anterior chamber with 0.05% benzalkonium bromide solution can build animal model of bullous keratopathy successfully;the implantation of slow release for bFGF micropellets to cornea propria in rabbit with bullous keratopathy can induce rapidly corneal neovascularization. Moreover, the clinical symptoms of pathological cornea relieve gradually following with the vegetation of CNV. So this experim- ental method may provid another route for the treatment of partial bullous keratopathy.
|
PDF Full Text Request