Identification Factor XIIIA Subunit During The Different Periods Of Deep Venous Thrombosis In Rats With Flow Cytometry

Objective:The objective is to identify the expression of FXIIIA on the surface of perpheral blood nucleated cells at the different time of deep venous thrombosis(DVT) in rats.Our work focus on the expression of FXIIIA on the membrane mentioned above in acute thrombosis.Then,to better understand the internal situation of thrombi,the ways to crack the thrombi in vitro were also discussed.Methods:1 78SD rats were randomly divided into DVT(n=36),and sham group(n=36),normal group(n=6).Furthermore,DVT group are divided into six subgroups,according to different duration of thrombi and/or emboli(2hours,4hours,12hours,24hours,7days and 14days) knowed as DVT2h(D2h),DVT4h(D4h),DVT12h(D12h),DVT24h(D24h), DVT7d(D7d),DVT14d(D14d) subgroup(n=6).An additional 36 rats underwent sham operations(six subgroups) corresponding to DVT subgroups as described above(n=6).Nomal groups had six groups(n=6).A rat model of thrombosis in femoral vein was established by the indwelling blood vessel clip to reduce blood flow.2 After the thrombi was cracked by trypsin,its liquid were collected and stained by indirect fluorescent antibody test(FITC) before being detected by flow cytometry(FCM).3 Artery blood and vein blood were extracted at the different time of DVT, then the blood were hemolyzed and stained by indirect fluorescent antibody test(FITC) and detected by flow cytometry(FCM).Results:1 There were remarkable differences about the expression of FXIIIA on the membrane above in D24 hours(actue DVT).2 the length of thrombi were increased from D2h to D24h,consistent with enhancement of FXIIIA.3 There were no remarkable differences between the artery blood and vein blood on the expression of FXIIIA.Conclusions:1 The expression of FXIIIA on the surface of nucleated cells was a hallmark of newly formed thrombi in vivo.2 The cells which were cracked by trypsin in vitro had good shape and a large number ones. This method is good to a better understanding of the internal situation of thrombi.3 There were no remarkable differences between the artery blood and vein blood on the expression of FXIIIA in nomal and DVT groups...