Lymphocyte Subpopulations And Cyclooxygenase-2 Expression In Nasopharyngeal Cancer

Nasopharyngeal carcinoma is a malignant tumor which derived from side and crest of nasopharyngeal; it has high mobility in Asia. It is also one of the highest mobility malignant tumors. Men is the main, the ordinary age is : 40-50; the second : 30-39 and 50-59. Now nasopharyngeal carcinoma is related to heredity,Epstein-Barr virus,environment, especially Epstein-Barr virus.In nasopharyngeal carcinoma, low differentiation squamous cell carcinomas can be seen usually. Radiotherapy is the first choice , during the radiotherapy,we can also chose chemotherapy and the Chinese medical science and traditional Chinese medical . We can adopt surgery if pathological changes are left after three month. Although the rate of effective controlling is 85%, but radiotherapy can lead the complication : the membrane of oval cavity dry, loss of hearing and secretors toasts media ; lingering effects of brain and the brainstem and vertebra. With the side effect of radiotherapy and chemotherapy,it affects living quality of patients. Radiotherapy is restricted in clinical. with the development of tumor immunitiology, Tumor composite treatment plays an important role. Here comes new progress for example adoptive cell therapy, another is target treatment. Adoptive cell therapy means, we can put activity immunity cell into the patient to kill the tumor. It is closed to cell immunity. With the development of immunology, people come to know that the disease is closed to immunity. Immunity plays an important role in cancer. Many articles have been reported with flow cell cytometry that CD3~+↓,CD4~+↓, CD4~+ / CD8~+↓obviously in malignant tumor. It points out that the function of cell immunity of malignant tumor goes down. This experiment We focus on the change of lymphocyte subpopulations in nasopharyngeal carcinoma, to learn the immunity answer to it. This is better to Adoptive cell therapy. It has positive clinical purpose.At present, the target treatment of COX-2 is a new hot focus. Cyclooxygenase-2 is one of important enzymes during cyclooxyge transform into prostate simple. It is associated with cell proliferation and differentiation, tumor infiltration and metastasis, angiogenesis. It can not be detected normally, but IL-2,TNF-αwhich CD4 secreted can up regulate the expression of COX-2.Our study detects the expression of T lymphocyte subpopulations and COX-2 in nasopharyngeal carcinoma and referent the results about COX-2 of group mates to analyze their relationship, which can provide us with available information for the target treatment of nasopharyngeal carcinoma.Objective: We adopt Immunohistochemistry method to check the expression of T lymphocyte subpopulations and COX-2 about nasopharyngeal carcinoma and the contrast. Explore the correlation of T lymphocyte subpopulations and COX-2 expression. It is important for further study the relationship between COX-2 and cell immunity, it provides available experiment for bases for adoptive cell therapy treatment and target treatment.Method: Nasopharyngeal carcinoma with clear pathological diagnosis 45 patients from The Second Affiliate of Dalian Medical University between 2002-2007.Barbarism cancer 27, scale 18, 32 lymph transfer, and 13 non-lymph transfer, nasopharyngeal with chronic nasopharyngitis 33 is on the contrast. Immunohistochemistry method was used to detect CD4~+, CD8~+, CD3~+, CD20~+ and COX-2 in canceration .Analyze the relationship between COX-2 and CD4~+, CD8~+, CD3~+,CD20~+.The experiment results were analyzed with X~2 test and Spearman by SPSS 13.0 statistic software, and P<0.05 was used as the criteria of marked differentia.Result:1.The positive rates of CD3+: the positive rate in nasopharyngeal carcinoma is 53.34%, including 13cases of +(28.89%),7 cases ++(15.56%) and 4 cases of +++(8.89%); the positive rate in nasopharyngitis is 81.82%, including 13cases of +(39.39%), 5 cases of ++(15.15%) and 9 cases of +++(27.27%).The positive rate in nasopharyngeal carcinoma significantly lower than that in nasopharyngitis(χ~2=8.96, P=0.030).2. The positive rates of CD4~+: the positive rate in nasopharyngeal carcinoma is 20%, including 9 cases of +(20%); the positive rate in nasopharyngitis is 42.42%, including 14cases of +(42.42%). The positive rate in nasopharyngeal carcinoma significantly lower than that in nasopharyngitis(χ~2=4.60, P=0.032).3. The positive rates of CD8~+: the positive rate in nasopharyngeal carcinoma is 77.77%, including 24cases of +(53.33%),9 cases ++(20.00%) and 2 cases of +++(4.44%); the positive rate in nasopharyngitis is 78.79%, including 22 cases of +(66.67%),4 cases of ++(12.12%) and 0 cases of +++(0%). There is no significant difference between these two groups(χ~2=3.51, P=0.319).4. The positive rates of CD20+: the positive rate in nasopharyngeal carcinoma is 48.88%, including 12cases of +(24.44%),6 cases ++(13.33%) and 5 cases of +++(11.11%); the positive rate in nasopharyngitis is 63.63%, including 10cases of +(30.30%), 7 cases of ++(21.21%) and 4 cases of +++(12.12%). There is no significant difference between these two groups(χ~2=1.89, P=0.595).5. The positive rates of COX-2: the positive rate in nasopharyngeal carcinoma is 86.67%, including 20 cases of +( 44.44%) ,12 cases ++( 26.67% ) and 7 cases of +++ ( 15.56% ) ; the positive rate in nasopharyngitis is 93.94%, including 6 cases of +(18.18%),11 cases of ++( 33.33% ) and 14 cases of +++ ( 42.42% ) .The positive rate in nasopharyngeal carcinoma significantly lower than that in nasopharyngitis(χ~2=10.31, P=0.016).6. Spearman correlation analysis was used to explore the correlation of COX-2 to CD3, CD4, CD8, and CD20 in nasopharyngeal carcinoma. The positive expression of COX-2 was correlated to that of CD4(R=0.464,P=0.023) ,but was not correlated to CD3, CD8 and CD20 ( R=0.245, P=0.249; R=-0.102, P=0.634; R=0.000, P=0.998)Conclusion:1. The number of CD3+ cell is more in nasopharyngeal carcinoma than in nasopharyngitis, with no significant difference about CD20~+ cells, which indicates that there is immunity dysfunction in nasopharyngeal carcinoma, especially cell immunity dysfunction.2. The number of CD3, CD4 in NPC is less than that of in CN, but that of CD8 between these two groups shows no difference. It makes clear that declining of the ratio of CD4~+/ CD8~+ maybe correlated with the local immunity dysfunction in pharynx nasalize.3. The positive rates of COX-2 expression are correlated to CD4, which indicates that CD4 can regulate the expression of COX-2 by some way.