Study On Hepatitis C Virus Genotype And Its Clinical Significance In Dalian

The infection of Hepatitis C Virus (hereinafter referred to as HCV) is distributed throughout the world, around 170 -200 million people are infected by HCV globally, about 42 million people in China are infected by HCV, which account for 3.2% of the total population. Being infected by HCV, 80% of those would be transformed to chronic persistent hepatitis, if the proper treatment is not available, after 10 to 20 years, 10%-30% of those who suffered chronic persistent hepatitis would be transformed to cirrhosis, and 1%-3% of those would be transformed to primary carcinoma of liver. It is discovered as through observation that genotype attached great significance in the process of HCV epidemic survey, the research of toxicity strength in virus, the development of specific vaccine for different sub-genotype virus, the nature process of virus infection and the function made in the course of illness due to constant infection, evolvement progress of virus, the mutual reaction between virus and host and clinical treatment effect.Objective:In order to find out the distribution and prevalence of HCV genotype in Dalian, Liaoning province, and make a research on the connection of HCV genotype and hepatitis C in terms of infected approaches, gender, age, inflammation activity, the degree of liver disease. It would be in an effort to provide substantial basis on the diagnosis and prevention of HCV. Therefore, it attaches great significance to the development of HCV diagnosis reagent and vaccine in Dalian in the years ahead.Methods:To select outpatient and inpatient with total 85 cases in The Second Affiliated Hospital of Dalian Medical University during the period from January, 2006 to November, 2007. To process with HCV gene classification to the 85 cases which have anti-HCV positive chronic hepatitis C, hepatitis C cirrhosis, hepatitis C cancer respectively by means of PCR and RELP. In those 85 cases, male/female: 45/40, age range: 17-96, average age is 50.94. (The diagnosis criteria to those cases complied with the standard mapped out in national hepatitis seminar held in Xian in September of 2000).Results:1. In those 85 anti-HCV positive cases, detection ratio of HCV RNA are 61cases, which account for 71.76% (61/85). In those 85 anti-HCV positive blood serum, 61cases are detected with 1b and 2a, 32 cases with 1b which account for 52% (32/61), 29 cases with 2a which account for 48%(29/61).2. With regard to the analysis of HCV genotype and infected approaches, being discovered that in those 61 cases with HCV RNA positive, 32 cases are hematogenous infection, 29 cases are emanatory infection, compared with the two figures, there are no distinct differences on structure of gene classification(p>0.05).3. In regard to the analysis in comparison with HCV genotype and age, the average age for 1b is around 48.16±18.15, the average age for 2a is around 55.69±13.30, being tested by t, there are no distinct differences between age and genotype(p>0.05).4. In regard to the analysis in comparison with HCV genotype and gender, 32 cases with 1b( male/female:18/14), 29 cases with 2a (male/female: 16/13), being tested by x2,there are no distinct differences between gender and genotype(p>0.05).5. With regard to the analysis in comparison with HCV genotype and ALT as well as TB, the fix quantity of ALT and TB of the genotype 1b and genotype 2a, the mean value of ALT on 1b and 2a are 111.83±178.43 U/L, 137.46±163.76 U/L respectively, the mean value of TB on 1b and 2a are 18.45±11.48μmol, 23.38±15.12μmol respectively. Being tested by t, there are no distinct differences between genotype and ALT as well as TB (p>0.05). 6. In the 32 cases with HCV 1b, 23 cases are chronic persistent hepatitis, 6 cases are cirrhosis, and 3 cases are liver cancer. In the 29 cases with HCV 2a, 25 cases are chronic persistent hepatitis, 3 cases are cirrhosis, and 1case is liver cancer. Being tested by x 2 ,there are no distinct differences among those who suffered hepatitis with HCV infection at various degree(p>0.05).Conclusion:1. Detection ratio of HCV RNA accounts for 71.76%, which matches with the medical literature published from home and abroad. Detection ratio of HCV RNA for those who suffered hepatitis C and cirrhosis is relatively higher, and detection ratio of HCV RNA for those who suffered liver cancer is relatively lower.2. There are two HCV genotypes in Dalian, province, namely, 1b and 2a, which matches with HCV genotype in China. In Dalian, 1b which account for 54% and 2a which account for 46% are equivalent to each other.3. There are no distinct differences between 1b and 2a in terms of gender, age, infected approaches, ALT and TB.4. It is not discovered that there are any differences between 1b and 2a because of the severity degree of hepatitis.