Study Of Prevation Effect Of Cuncumin On Bacterial Sepsis

Background and ObjectiveSystemic inflammatory response syndrome (SIRS) is triggered by bacteria and bacterial components generally associated with an infection (sepsis) ,can developed into septic shock ,MODS and so on. Unfortunately, many experimental inflammatory antagonist-based therapies have failed in sepsis trials, and there are currently no effective anti-sepsis drugs in clinical use. Curcumin (Cur) has the effect of antagonizing of the inflammation induced by LPS.This subject will observe the prevation effect on the sepsis mice infected by E.coli,and study its mechanism on the level of molecular.Methods(1) Murine RAW264.7 cells were cultivated and induced by LPS to release TNF-αand NO, and to investigate effect of Cur on these cytokines and the dose-response relationship;(2) To establish murine sepsis models, then the protection of Cur on the mice was observed;(3) Effect of Cur on NF-кB activation within RAW264.7 cells stimulated by LPS was observed using western blot;(4) Effect of Cur on the expression of HSP70 within RAW264.7 cells was observed by western blot.Results(1) In vitro,treatment of murine macrophage RAW264.7 cells with Cur potently inhibited the release of TNF-αand IL-6 induced by LPS in dose -dependent manners.(2) We found that 100, 200mg/kg of Cur could protect mice from lethal challenge by LPS and also could decrease serum TNF-αin mice injected with lethal doses of LPS.(3) Furthermore, Cur inhibited the NF-кB shifting to nuclear stimulated by LPS.(4) Cur could induce the expression of Hsp70 in RAW264.7 cells in dose- dependent and time-dependent manners.ConclusionsOur results demonstrated that Cur-mediated protection of lethal challenge by LPS and the protective effect on mice challenge by active E.coli was associated with the reduction of proinflammatory cytokine release such as NF-кB and the activation of the expression of Hsp70 protein.