Transforming Growth Factor-β1 Enhanced Vascular Endothelial Growth Factor Synthesis In Mesenchymal Stem Cells

Objective:To explore the effect of TGF-β1 on VEGF synthesis in MSCs and the mechanisim.Methods:Bone mesenchymal stem cells were isolated from male SD rats and cultured.Cells of passage 3 to 5 were used for the experiment. BMSCs were treated with differents concentrations of TGF-β1,and VEGF and the mRNA level were tested.Ly 294002 and U0126 were used to check whether Akt and Erk1/2 signal were involved in this process.Results:The exposure of MSCs to TGF-β1 stimulated the synthesis of VEGF.Meanwhile,TGF-β1 stimulated the phosphorylation of Akt and extracellular signal-regulated kinase 1/2(ERK1/2).Moreover,Ly 294002,a specific inhibitor of phosphatidylinositol-3-kinase(PI3K) /Akt significantly attenuated the VEGF synthesis stimulated by TGF-β1. Additionally,U0126,a specific inhibitor of ERK1/2,also significantly attenuated the TGF-β1-stimulated VEGF synthesis.Conclusion:These results indicated that TGF-β1 enhanced VEGF synthesis in MSCs,and the Akt and ERK1/2 activation were involved in this process.