Expression And Significance Of Tissue Factor In Acute Pancreatitis.

Objective: To observe the changes of tissue factor (TF) in rats with acute pancreatitis, and to explore relationship between TF with acute pancreatitis ,and reaserch the function of TF, which as an important coagulation factor and inflammatory in the procedure of acute pancreatitis.Methods: Ninety adult Wistar rats were randomly divided into three groups: Control group (C), Moderate group (M), and Severe group (S). Ten rats of each groups were killed at 1, 6, 12h points after model was established and pancreatic tissue was collected. Aurocholic acid was injected into into bile duct to establish pancreatitis model. Reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemistry(IH) were used to detect the lever of TF mRNA and protein of TF in rats with acute pancreatitis. The relationship between the expression of TF and the pathological changes was analyzed statistically.Results1. After injecting taurocholic acid, we can see directly edema, bleeding and necrosis in pancreas, there were different changes with injecting different concentration. Under microscope we can find the same changes.2. The serum amylase was higher in group M and group S than group C, it showed a significant difference(P<0.05); but it was no difference in group M and group S (P>0.05).3.The level of TFmRNA expression was increased significantly in group S at 1hour point compared with that in group C(P<0.05) ,and maintained at high levels up to 6hour point after model was made. Similarly, the expression of pancreas TF protein was also markedly increased in group S 1hour point after model was made as compared with that in group C.Conclusions As a coagulation factor and inflammatory factor, TF expressed obviously in acute pancreatitis, and played a important role in the procedure of acute pancreatitis.