A Study On Epstein-Barr Virus In Nasopharyngeal Carcinoma Diagnosis And Therapy

Background and objective:Epstein-Barr virus(EBV)is closely associated with nasopharyngeal carcinoma(NPC),and EBV-VCA/IgA is served as a biomarker of NPC in early stage.Recently,it was reported that EBV-DNA quantitive assay in plasma had some clinical significance to NPC early diagnosis.It has not been illuminated whether EBV-DNA was associated with NPC tumor size. In this present,the dynamic study of EBV-VCA/IgA and EBV-DNA was conducted in treatment progress,and the association of EBV-DNA and NPC tumor size was investigated.Methods:1.To definite the NPC patients with EBV.The suspected NPC outpatients in ENT of Xiangya Hospital were pathologically diagnosed and EBV-LMP1 was detected in biopsy tissues by immunohistochemistry from May 2006 to January 2008.2.To determine patients and collect samples.NPC patients were as the objects if the NPC patients with positive EBV agreed to join in experiment group.The plasma and serum were collected at pre-treatment and one,two,three,four,five,six,seven weeks and three months after treatment.3.The plasma EBV-DNA concentration was analyzed by using fluorescent quantitative nested-PCR technique in Mx3000p.The median concentration was calculated and dynamic pictures were drawn.4.The levels of EBV-VCA/IgA were detected through ELISA assay and dynamic pictures were drawn by OD.5.The NPC tumor size was measured using magnetic resonance imaging technology.Results:1.The results of immunohistochemistry showed that LMP1 protein expressed in cytoplasm or cytomembraneo The positive rate was 42.5% (34/80).2.EBV-DNA was detected in 27/28 NPC patients plasma,and positive rate was 96.4%.The median concentration was 1800 copies/μ1 (range:0-22100).Among 30 cases,EBV-DNA were declining in 18 cases and increasing in 9 cases.3.24/28 cases were detectable for serum EBV-VCA/IgA.During treatment EBV-VCA/IgA were declining in 17 cases and increasing in 6 cases.4.By analyzing relationships between EBV-VCA/IgA or EBV-DNA and tumor size,we found EBV-DNA were associated with tumor volume to a certain extent.Conclusions:1.The fluorescent quantitative nested-PCR was established for detecting EBV-DNA by combining the nested PCR with the fluorescent quantitative PCR.2.It was clear that the EBV-DNA was closed related with curative effect and tumor burden through dynamic study.3.The EBV-VCA/IgA and EBV-DNA could be used to screen NPC patients and had clinical significance.