| Objective:The expression of vasoactive intestinal peptide(VIP)in the different impaired cartilage of osteoarthritis of human was studied to approach the correlation and possible involvement of VIP in pathogenesis and progression of osteoarthritis as to give a theoretical evidence for prevention and treatment for OA..Methods:Human cartilages were obtained at arthroplasty from 26 patients with OA.Corresponding articular cartilages were obtained at arthroplasty or amputation for trauma,tumour or infection from 10 patients with no history of arthritis.Then collect 47 cartilage specimens from OA and normal joint,all the specimens were fixed,decalcified,embedded in paraffin and cut into slices.Each slices sample was stained with HE and Safranin O,on the basis of improved Mankin pathological score to divide into four groups,normal group 9 specimens(group A),mild impaired group 10 specimens(group B),medium impaired group 15 specimens (group C),severe impaired group 13 specimens(group D).Meanwhile,the slices were treated by immunohistochemical reaction of VIP and dealed with micrograph analyze instrument(MIAS system).Each slice was randomly mensured 4 high fields of eyeshot,and made semi-quantitative analysis of average optical density(AOD).The data was analyzed with Spearman rank correlative analysis and analysis of variance,performed in SPSS Star software.P values less than 0.05 were considered statisticaly significant.Results:1.VIP positive nerve fibers exist in normal and OA human cartilage in all layer,the pattern of VIP expression are scattered into the matrix. Some terminated in the intermaediate layer matrix,others ran into the deep radial layer and terminated around cartilage cells.There are no significant differences between each group.2.The AOD of VIP in the group A is 0.2603±0.0396,group B 0.2412±0.0341,group C 0.1881±0.0444 and group D 0.1638±0.0385。There are significant differences between osteoarthritis group and nomal group(p＜0.05).There are no significant differences between group A and group B,While there are significant differences between group A and group C&D(p＜0.01).According to Spearman rank correlation test,the AOD of VIP have direct correlation with the improved Mankin pathological score(r=-0.896,p＜0.05).Conclusion:1.The first time discovered that VIP positive nerve fibers exist in normal human cartilage.2.VIP low expression is one of important pathogenesis in OA.3.With the expression of VIP decreased,the cartalege of OA impaired increases by degrees.
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