The Study Of Transbone And Chondrol Iontophoresis Of Vancomycin

Objective: to apply transdermal iontophoresis of vancomycin to infectious disease in orthopedics. To study the feasibility of transbone or chondro-iontophoresis or transdermal-bone iontophoresis of vancomycin. To research the antibacterial stability of vanconmycin after iontophoresis and the toxicity of vanconmycin to cartilage cell, and get invaluate of how deep the drugs could be.Methods: calculate the regressive equation based on the standard curve of vancomycin. Then devide the whole experiment into 3 parts:In chapter one,we devide 13 adult rabbits into 3 groups. All the 3 rabbits in first group used for in vitro qualitation. To investigate the depth that methyl blue could reach in tibia, on which 1.0mA, 20:1 impulsive current is given for 1 hour. 10 rabbits in group 2 used to investigate the qantitation of vancomycin in the tibia, on which 1.0mA 20:1 impulsive current is given for 1 hour. For each rabbit, one tibia is used for experimental group, the other is then used for control group, in which vancomycin is diosmosised freely. Then measure the concentration of vancomycin quantitatively. Compare the difference of the two management by two independent sampler t test.In chapter two, we divided 15 rabbits into 2 groups for in vivo transbone and chondro-iontophoresis. All the 7 rabbits' hind limb in the first group shaved. Hypodermic injection for 40,000u/ml vancomycin. For each rabbit, one tibia is used for experimental group, the other is then used for control group, in which vancomycin is diosmosised freely. Then measure the concentration of vancomycin quantitatively. Compare the difference of the two management by two independent sampler t test. Rabbits in group 2 are used for chondro-iontophoresis experiment, to investigate the antibacterial stability of vanconmycin after iontophoresis and the toxicity of vanconmycin to cartilage cell, and get invaluate of how deep the drugs could be.In chapter three, we chose 7 rabbits to manage experimentation of in vivo transdermal-bone iontophoresis. Also tried to manage diosmosis vancomycin into tibia bone from the skin. Then gain the tissue under the skin, cortical bone, and marrow to measure the concentration of vancomycin quantitatively.Results: we got satisfactory results in all experiments in chaper one and chapter two(P < 0. 01), except for marrow. Result of experiments in chapter three shows a negtive difference between experimental group and control group.Conclusions: The study confirmed that vancomycin could not only be diosmosised transdermal, also could be diosmosised transbone and cartilage by iontophoresis. Furthermore, the repressing bacteria effect of vancomycin will not be changed by iontophoresis. But we failed to confirm that vancomycin could be diosmosised into tibia bone from skin in ordinary state. Whether there is process which could improve the osmolarity of vancomycin and/or improve the permeability of skin, needs further research.