The Research On Microangiopathy And Immune Function In Type 2 Diabetes Mellitus Patients With Black Patches On Pre-tibia

Objective:The present study was to investigate the correlation among black patches on pre-tibia,immune function and microangiopathy.Methods:64 T2DM patients and 48 normal subjects were enrolled in this study. T2DM patients were divided into two groups:black-patch group and non-black-patch group.Percentage of CD3+ to total lymphocytes was measured through flow cytometry,so were the percentages of CD4+ and CD8+ to T lymphocytes;The concentration of MMP-9 was measured through ELISA.Results:(1)The percentage of CD3+ to total lymphocytes in T2DM group was not significantly different from normal group(P＞0.05);The percentage of CD4+ to T lymphocytes in T2DM was significantly higher than that in the normal(P＜0.01); The percentage of CD8+ to T lymphocytes in T2DM group was significantly lower than that in normal group(P＜0.01);The ratio of CD4+/CD8+ in T2DM were significantly higher than that in the normal(P＜0.01);IgG and CIC were significantly lower in T2DM than those in normal group(P＜0.01)(2)Incidence of chronic complications,especially microangiopathy,in the black-patche group was significantly higher than that in the non-black-patch group(P＜0.05)(3)There was no significance between black-patch group and non-black-patch group in the percentage of CD3+ to total lymphocytes(P＞0.05);The percentage of CD8+ to T lymphocytes in the black-patch group was significantly lower than that in the non-black-patch group(P＜0.05):Both of the the percentage of CD4+ and the ratio of CD4+/CD8+ in the black-patch group were significantly higher than those in the non-black-patche group(P＜0.01,P＜0.05 respectively);IgG,CIC and IgA were higher in the black-patch group than those in the non-black-patch group,but there were no significance(P＞0.05)(4)The percentage of CD4+ was positively correlated with IgG and IgA(r=0.381,P＜0.05;r=0.396,P＜0.01,respectively);The percentage of CD8+ was negatively correlated with IgG and IgA(r=-0.434,P＜0.05; r=-0.499,P＜0.05 respectively);The ratio of CD4+/CD8+ was positively correlated with IgG and IgA(r=0.407,P＜0.05;r=0.411,P＜0.05 respectively)(6)The concentration of MMP-9 in T2DM was significantly higher than that in normal group(P＜0.05); The concentration of MMP-9 in the black-patch group was higher than that in the non-black-patch group,but there was no significance(P＞0.05).Conclusion:(1)There was a higher incidence of microangiopathy in type 2 diabetes patients with black patches,indicating that the incidence of black-patches was closely correlated with that of diabetic microangiopathy,so the T2DM patients with black-patches should be screened for the existence of microangiopathy(2)The increase of CD4+ and the decrease of CD8+ in T2DM patients,especially those with black patches,suggested that there was a disorder of immune function in T2DM and the disorder was more severe in T2DM with black patches(3)Black patches on pre-tibia could be a dermatic sign of immune disorder in type 2 diabetes mellitus.We should pay more attention to the immune function of T2DM patients with black patches and treat the immune disorder as early as we can.