Research About Adipocytokines And Metabolic Syndrome

Objective Metabolic syndrome is incorporates into a single entity, insulin resistance and its associated cluster of related cardiovascular metabolic risk factors including essential hypertension, central obesity, hyper-triglyceridemia, hypo-high density lipoprotein cholesterol, impaired glucose tolerance and type 2 diabetes mellitus. Recent studies show that adipose tissue is not simply storage of energy, but is an active endocrine organ. These are achieved predominantly through release of adipocytokines, which include several novel and highly active molecules released abundantly by adipocytes like leptin, resistin, adiponectin or visfatin, as well as some more classical cytokines released possibly by inflammatory cells infiltrating fat, like tumor necrosis factor-a(TNF-a), interleukin-6(IL-6), interleukin-1(IL-1), monocyte chemo attractant protein (MCP-1), C reactive protein(CRP), et al, and some thrombogenesis factors, such as plasminogen activator inhibitor-1(PAI-1), fibrinogen, et al. The adipokine secreted by adipose tissue, such as adiponectin, leptin, resistin, play important roles in the pathogenesis of metabolic syndrome. This research is to investigate the change of adipokine levels in patients with metabolic syndrome, and their relationship with the components of metabolic syndrome. The aim of this study is also to detect the relationship between serum adiponectin, leptin and body mass index, waist circumference, waist to hip ratio, systolic blood pressure, diastolic blood pressure, fasting blood glucose(FBG), serum total cholesterol(TC), triglyceride (TG), high density lipoprotein cholesterol(HDL-C), low density lipoprotein cholesterol(LDL-C).Methods: Based on standards of NECP-ATPIII, 36 MS patients who were first diagnostic in the health examination were selected as MS group [20 women and 16men; mean (±SD) age, 59.2±8.2 years] and 37 healthy subjects as control group [18 women and 19 men; mean (±SD) age, 60.3±8.5 years]. After measured the anthropometric parameters: the height, weight ,waist circumference, hip circumference, the waist to hip ratio, systolic blood pressure and diastolic blood pressure, the biochemical parameters of MS group and healthy control group were collected: the fasting blood glucose, serum total cholesterol, triglyceride, high density lipoprotein cholesterol, low density lipoprotein cholesterol were determined. The serum adiponectin and leptin levels were measured with enzyme linked immunosorbent assay (ELISA). All analyses were performed with the use of SPSS software (version 13.0). P<0.05 shew statistical significantly different.Result:1. The adiponectin level of MS patents was lower significantly than healthy subjects (1.71±1.73mg/L vs. 2.80±1.80mg/L, P<0.05). It is not value significantly different between MS patents and healthy subjects in serum leptin level (0.94±0.64ug/L vs. 1.22±0.83ug/L, P>0.05).2. In MS group, serum adiponectin concentrations were negatively correlated with the total cholesterol (r=-0.432, P=0.009), LDL-C (r=-0.404, P=0.014). In male group, serum adiponectin concentrations were negatively correlated with the total cholesterol (r=-0.369, P=0.027), LDL-C(r=-0.381, P=0.022), and body mass index (r=-0.370, P=0.026). In female group, serum adiponectin concentrations were negatively correlated with systolic blood pressure (r=-0.348, P=0.032), total cholesterol (r=-0.322, P=0.049). Controlling for the gender and age in MS group, adiponectin levels was negatively correlated with total cholesterol (r=-0.435, P=0.010) and LDL -C levels(r=-0.435, P=0.010).3. There is no relationship between serum leptin level and other determinations.4. The stepwise linear regression between serum adiponectin and other determinations shows that there is linear relationship between serum adiponectin and total cholesterols.Conclusions:1. The adiponectin level decreased in MS patients who were first diagnostic in the health examination.2. The linear correlation between serum adiponectin and other determinations: in MS group, serum adiponectin concentrations were negatively correlated with the total cholesterol and LDL-C; in male group, serum adiponectin concentrations were negatively correlated with the total cholesterol, LDL-C, and body mass index; in female group, serum adiponectin concentrations were negatively correlated with systolic blood pressure, total cholesterol. Controlling for the gender and age in MS group, adiponectin levels was negatively correlated with total cholesterol and LDL -C.