Relative Study Of ACAID In The Rejection Of Corneal Allograft In High-risk Eyes

Objective: To investigate the method of the animal model of anterior chamber associated immune deviation by soluble antigen. To investigate the effect of donor-specific anterior chamber-associated immune deviation in the rejection of corneal allograft in high-risk eyes.Methods:Corneal alkali burn injuries model were induced by New Zealand white rabbits. New Zealand white rabbits were randomly allocated into induced group and control group. Bovine serum albumin was inoculated into the anterior chamber of induced group. Balanced salt solution was inoculated into the anterior chamber of control group. Then induced delayed type hype sensitivity. After seven days,recipients were immunized with bovine serum albumin in complete Freund' s adjuvant(1:1). One-week later,antigen-specific delayed-type hypersensitivity was evaluated. Corneal alkali burn injuries model were induced by New Zealand white rabbits. These rabbits were randomly allocate d into 4 groups:A:normal cornea of keratoplasty group, B: alkali burned corneal keratoplasty group, C: normal cornea induced by ACAID keratoplasty group, D: alkali burned corneal induced by ACAID keratoplasty group. The left cornea of B and D groups were burned by alkali, and after that 1 month orthotopic keratoplasty was performed.2 weeks before keratoplasty, donor-specific ACAID was induced by inoculating soluble antigen into recipient's anterior chamber in C and D groups, whereas balanced salt solution was inoculated into the right eye anterior chamber of A and B groups. Graft mean survival times, neovascularization and rejection index were determined. The cornea were performed with HE(Hematoxylin eosin)technique.Results:Neovascularization and on central corneal(15.11±1.45)day. Control group elicited an intensive antigen-specific DTH. By contrast,the induced group failed to display antigen-specific DTH. The graft of A and C groups were survival long-term. The graft mean survival times of B groups was(25.13±0.64)day. The graft mean survival times of D groups was(38.25±1.28)day. The alkali burned corneal keratoplasty group, there were inflammatory cells had spread into the corneal when rejection occurred. There were even more lymphocytes and macrophagocytes in the alkali burned corneal keratoplasty group. Corneal allografts survival of D group was enhanced (P<0.05), comparing with that of B group.Conclusion:Corneal alkali burn injuries model is a good method. The method of the animal model of anterior chamber associated immune deviation by soluble antigen is a good way to induce ACAID. Induction of donor-specific ACAID effectively prolongs corneal allograft survival time in alkali burned high-risk eyes.