The Study About The Effect Of Xuefuzhuyu Decoction On Human Mesangial Cells Regulation In A High Glucose Environment

Purpose:To investigate the effects and mechanisms of XueFuZhuYu Decoction in the patients with diabetic nephropathy.Meodsth:Using Mesangial cell(MC)cultivating technology,serum pharmacological technology,The effects of XueFuZhuYu Decoction on the Secretion transforming growth factor-β1(TGF-β1)laminin(LN)of the cultured human mesangial cells under high glucose condition were observed in vitro.7 healthy volunteers who have similar age and height took the xuefuzhuyu decoction for 7 days.Then get the serum containing xuefuzhuyu decoction solution.Blank serum was taken as control.The primary cultured human mesangial cells was resuscitated and cultured with conventionally. Then they were passaged.Cells were cultured separately in normal glucose, high glucose,high glucose with blank serum,high glucose with low xuefuzhuyu decoction serum,high glucose with medial xuefuzhuyu decoction serum,high glucose with high xuefuzhuyu decoction serum,and Simvastat-treated high glucose group.Give respectively each one educates liquid.Educates respectively 24,48,the 72 hs withdraws to educate liquid top pure-20℃conservancy back up.The adoption an anti-clips the heart ELISA method measurement transforming growth factor-β1(TGF-β1)contain the deal.The adoption RIA method measurement layer glues to connect laminin(LN)contains the deal.And compute 24 The hour represses the rate.Results:Compared with the A group,the mesangial cells secret transforming growth factor-β1(TGF-β1)laminin(LN)Increment obviously in the B group(P＜0.01).Compared with the B group,the Secretion transforming growth factor-β1(TGF-β1)and laminin(LN)of mesangial cells were not inhibited in the C group(P＞0.05).The Secretion transforming growth factor-β1(TGF-β1)and laminin(LN)of mesangial cells were all inhibited in the F,E and Fgroups compared with the B group(P＜0.01) This efect is in a dose-dependent manner.Compared with the Bgroup,the Secretion transforming growth factor-β1(TGF-β1)and laminin(LN)of mesangial cells were also inhibited in the G group(P＜0.01).There was obvious diferrence in the E and G group(P＞0.05)Conclusion:(1)MC is the important target cell of xuefuzhuyu decoction.It could be one of the important mechanism of xuefuzhuyu decoction treating Diabetic nephropathy.(2)Xuefuzhuyu decoction of the different density can repress the MC outside and high sugar in environment descends to educate to secrete transforming growth factor-β1(TGF-β1)and laminin(LN),and report measures dependence.(3)Xuefuzhuyu decoction represses Mesangial cells to secretes transforming growth factor-β1 (TGF-β1)and laminin(LN).That may be the part of function mechanism of xuefuzhuyu decoction repressing obviously the mesangial cells in high sugar to secrete cell fator and Extracellular Martrix(ECM)in cell.