The Study On The Influence Of NO,nNOS In The Brain And Ethology Changes Of PD Model Rats Thrugh Acupuncturing Baihui(DU20) Ming Men (DU4)

Objective:To study the possible mechanism of the bahui and mingmen acupuncture(BMA)in the treatment of Parkinson's desease(PD)Methord:C⁵⁷BL/6 mice were divided randomly into four group: control group,model group,BMA group and Madopar group.Mice in the model group,BMA group and Madopar group were injected with MPTP to establish PD animal model and then were treated with normal saline(NS), BMA and Madopar respectively.Animals were examined behaviorally with the pole test and the traction test.After the animals were killed,the content of NO and the cells amounts of nNOS in the substantia nigra(SN)of mice were examined.At the same time the morphology of neurons in the SN was observed with microscope.Result:1.The grades of the both pole and hang tests in the model group were lower remarkably compared with the normal group(P<0.05);The grades of the both tests in the BMA group and the Madopar group increased remarkably,compared with the model group(P<0.05);The scores of the pole test in the BMA group and Madopar group has little difference(P> 0.05).2.Dopaminergic neuron in the model group has irregular shape (polygon or long spindle)and unclear structure;The number of neurons in the model group decreased remarkably;The number of neurons in the BMA group and the Madopar group was close to the number in the control group.3.The content and amount of NO and nNOS in the SN of mice in the model group increased remarkably compared with the normal group (P<0.01);When treated with BMA and Madopar,the content and amount of NO and nNOS decreased remarkably compared with the model group (P<0.01);The content and amount of NO and nNOS in the BMA group and Madopar group has little difference(P>0.05)Conclusions:1.BMA could improve the dyskinesia of PD mouse model.2.BMA could fix the damage of dopaminergic neurons in the SN of PD mouse model.3.BMA has the similar therapic effect on PD as Madopar. 4.BMA can improve the metabolism of free radical and decrease poisonous function in mice brains.