The Study Of Zidian Shengyu Pulvis Affects On Costimulatory Molecules CD86 In Model Mice With CITP

Objective:To investigate the mechanism by which Zidian Shengyu Pulvis(ZSP)affects the development of CITP through observing changes of PLT number and the peripheral blood expression of CD86 in CITP model mice.Methods:1.The mice were divided into six groups:normal,model,hormone control,low dose of ZSP,middle dose of ZSP,and high dose of ZSP.2.The chronic ITP model was established by intraperitoneally injection of anti-BALB/c platelet serum(APS).3.The platelet number in mice was calculated by an automatic blood cell counter.The peripheral blood expression of CD86 in mice was measured by ELISA.The mice in treatment groups were gavaged with ZSP for 14 consecutive days starting 8 hours agter the 1st abdominal cavity injection of APS.Results:The PLT number in mice of the model group was lower than that in normal group,and the expression of CD86 in mice of the model group was statistically higher than that in the normal group(P＜0.05).Compared with the model group,mice in all the treatment groups showed statistically higher PLT number and lower CD86 expression levels(P＜0.05).No significant difference was observed among the middle dose of ZSP,high dose of ZSP and the hormone group.Conclusion:CD86 was expressed in peripheral blood at higher level in the ITP model mice.ZSP has functions of lowering peripheral blood expression of CD86 and increasing PLT number.It plays a role in the treatment of ITP by regulating immunological functions to reduce PLT destruction.