Experimental Study About The Effects Of Garlic Oil On Nonalcoholic Fatty Liver Desease In Rats

Objective:In this study,we established NAFLD model in rats fed with high-fat diet,and then based on this model,garlic oil was used as interventional agent to elucidate:1.the therapeutic effect of garlic oil in rats with NAFLD;2.the mechanism of garlic oil on NAFLD;3.compare the effect of garlic oil,dially trisulfide and Simvastatin in rats with NAFLD;The results of this study are expected to help to know the pathogenesis of NAFLD and provide new clinical therapeutic methods for NAFLD.Methods:1.ANIMAL TREATMENT80 male Wister rats were randomly divided into 7 groups:normal control group, model group,high-dose garlic oil group,mid-dose garlic oil group,low-dose garlic oil group,dially trisulfide group and Simvastatin group.Most of the rats except the normal control group were injected with a low dose of carbon tetrachloride one time on the first day of experiment.The rats of garlic oil groups were administered with garlic oil orally,40 mg/kg.bw,80 mg/kg.bw,120 mg/kg.bw respectively,while the normal control,the modle group,the Dially trisulfide group and Simvastatin group were treated with the same volume distilled water,corn oil,dially trisulfide and Simvastatin once a day.Fatty liver models were induced by chronically feeding with high fat diet(including:fat 20%,cholesterol 11%,sodium deoxycholate 0.2%, Propylthiouracil 0.2%,base forage 78.6%)for eight weeks.2.EXPERIMENTAL INDICESBody mass,liver weight,transaminase level,blood glucose,triglyceride(TG), total cholesterol(TC),insulin level,high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C),malondiaidehyde(MDA),superoxide dismut -ase(SOD),glutathione(GSH),glutathione peroxidase(GSH-Px),total anti-oxidative capacity(T-AOC),free fat acid(FFA)and liver histology were detected.Results:1.The rats of the normal control group are in good condition and their coat is glossy. The weight of other groups is decreased compared with the normal control group,and their coats are less glossy.The ratio of liver and body weight is increased in the model group compared with the normal control group(P<0.01).The ratio of liver and body weight of the high dose group,mid dose group,low dose group,dially trisulfide group and simvastati group are decreased compared with the model group.2.Serum biochemical results1)Glucose:the model group increased by 34.60%compared with the normal control group(P<0.01),the high dose group,mid dose group,low dose group,dially trisulfide group and simvastati group respectively decreased by 16.30%,15.99%,10.03%, 18.03%and 17.40%compared with the model group.2)ALT:the model group increased by 107.18%compared with the normal control group(P<0.01),the high dose group,mid dose group,low dose group,dially trisulfide group and simvastati group respectively decreased by 33.15%,32.28%,24.69%, 22.52%and 23.97%compared with the model group.3)TC:the model group increased by 84.28%compared with the normal control group (P<0.01),the high dose group,mid dose group,low dose group,dially trisulfide group and simvastati group respectively decreased by 19.45%(P<0.01),8.19%,4.78%, 3.07%and 18.09%compared with the model group.4)TG:the model group increased by 233.33%compared with the normal control group(P<0.01),the high dose group,mid dose group,low dose group,dially trisulfide group and simvastati group respectively decreased by 33.85%,59.51%,36.15%, 58.46%and 50%compared with the model group(P<0.01).5)LDL-C:the model group increased by 57.73%compared with the normal control group(P<0.01),the high close group,mid dose group,and simvastati group respectively decreased by 16.34%,13.07%,16.34%,58.46%and 50.00%compared with the model group.6)SOD:the model group decreased by 44.94%compared with the normal control group(P<0.01),the high dose group,mid dose group,low dose group,dially trisulfide group and simvastati group respectively increased by 58.30%,37.98%,26.09%, 68.79%and 70.20%compared with the model group.7.)MDA:the model group increased by 77.74%compared with the normal control group(P<0.01),the high dose group,mid dose group,low dose group,dially trisulfide group and simvastati group respectively decreased by 26.90%,22.90%,19.31%, 27.93%and 34.05%compared with the model group.8)FFA:the model group increased by 102.02%compared with the normal control group(P<0.01),the high dose group,mid dose group,low dose group,dially trisulfide group and simvastati group respectively decreased by 38.78%,37.76%,25.20%, 38.12%and 28.30%compared with the model group(P<0.01).9)GSH:the model group decreased by 31.18%compared with the normal control group(P<0.01),the high dose group,mid dose group,low dose group,dially trisulfide group and simvastati group respectively increased by 18.92%,17.11%,10.12%, 21.93%and 17.83%compared with the model group.10)T-AOC:the model group decreased by 47.81%compared with the normal control group(P<0.01),the high dose group,mid dose group,low dose group,dially trisulfide group and simvastati group respectively increased by 49.53%,48.10%,43.98%, 30.70%and 18.67%compared with the model group.11)AST and HDL-C of every group do not have significant difference.GSH-PX of the model group decreased by 29.55%compared with the normal control group (P<0.01),GSH-PX of other groups have no significant difference compared with the normal control group.3.Results of liver homogenate1)TC:the model group increased by 347.22%compared with the normal control group(P<0.01),the high dose group,mid dose group,low dose group,dially trisulfide group and simvastati group respectively decreased by 40.99%,34.78%,32.91%, 78.89%and 39.13%compared with the model group(P<0.01). 2)TG:the model group increased by 211.43%compared with the normal control group(P<0.01),the high dose group,mid dose group,low dose group,dially trisulfide group and slmvastati group respectively decreased by 39.00%,36.75%,24.51%, 42.03%and 47.48%compared with the model group(P<0.01).3)SOD:the model group decreased by 31.52%compared with the normal control group(P<0.01),the high dose group,mid dose group,low dose group,dially trisulfide group and simvastati group respectively increased by 36.90%,29.98%,28.79%, 41.39%and 34.01%compared with the model group(P<0.01).4)MDA:the model group increased by 189.21%compared with the normal control group(P<0.01),the high dose group,mid dose group,low dose group,dially trisulfide group and simvastati group respectively decreased by 27.36%,23.88%,18.16%, 26.37%and 30.85%compared with the model group.5)FFA:the model group increased by 383.29%compared with the normal control group(P<0.01),the high dose group,mid dose group,low dose group,dially trisulfide group and simvastati group respectively decreased by 67.67%,64.48%,54.33%, 61.83%and 60.18%compared with the model group(P<0.01).6)GSH:the model group decreased by 25.93%compared with the normal control group(P<0.01),the high dose group,mid dose group,low dose group,dially trisulfide group and simvastati group respectively increased by 13.90%,12.79%,4.03%, 15.03%and 17.45%compared with the model group.7)T-AOC:the model group decreased by 42.86%compared with the normal control group(P<0.01),the high dose group,mid dose group,low dose group,dially trisulfide group and slmvastati group respectively increased by 34.62%,28.85%,1.92%, 44.23%and 32.69%compared with the model group.8)GSH-PX of the model group decreased by 81.24%compared with the normal control group(P<0.01),GSH-PX of other groups have no significant difference compared with the normal control group.4.Pathologically,the livers of normal control group are in good condition.The livers of the model group presented severe hepatocyte steatosis.The liver of high dose garlic oil,dially trisulfide group moderated compared with model group. Conclusion1.Long-time feeding with high-fat and high-cholesterol diet can estabolished rat NAFLD model.,which exhibited as significant insulin resistance and lipid metabolic disorders.2.Garlic oil not only attenuated hepatocyte steatosis,but also meliorate liver inflammatory cell infiltration.3.Garlic oil can improve insulin resistance and lipid metabolic disorder,reduce liver weight and liver indices.4.Garlic oil enhanced the antioxidant capacity.It can increase the SOD,GSH and T-AOC level in serum and liver homogenate of rats,and even could decrease the MDA level in serum and liver homogenate.