Study On The HPLC Fingerprint Of Rhizoma Alpinia Ofificinarum And Initial Approach Of Galangin Absorption And Excretion In Vivo

In this article, we have established HPLC Fingerprint of Rhizoma Alpinia Ofificinarum with Chinese medicine finger printing technique, which have got the international social approval at the present stage. And we also approached the Metabolism in Vivo of Galangin which is the key component of Rhizoma Alpinia Ofificinarum.一,Study on the HPLC Fingerprint of Rhizoma Alpinia Ofificinarum and the Determination of GalanginObjective: To establish the HPLC fingerprint of rhizoma alpinia officinarum and to determine the content of galangin.Method: Separation was performed on Phenomenex—C18 (4.6mm*250mm, 5um) chromatographic column, acetonitrile-0.2% phosphoric acid as mobile phase with gradient elution . The detection wavelength was at 280 nm (that of galangin determination was at 235nm); analysis the data by the similarity of fingerprint software.Result: Confirmed nine common peaks in chromatograms of the 14 batches of samples and established the common pattern of them, while compared the content of galangin in each sample.Conclusions: Fingerprint with the determination of effective constituent could be used to control the quality of Alpinia ofificinarum Hanc availably.二,Initial approach of Galangin absorption and excretion in VivoObjective: To investigate the change of Rat's blood serum., urine and dejecta after being fed respectively Galanga Tables and Galangin.Method:①12 SD rats were randomly divided into two groups, being fed Galanga Tables and Galangin respectively, and fasted for 24 hours but drank freely. Collect Rat's blood serum., urine and dejecta before and after the study. All the samples were reserved under—4℃.The dejecta samples would be handled after extacting with 95% Alcohol.②56 SD rats were randomly divided into eight groups ,corresponding with eight blood points: 0.5h,1h,1.5h,2h,2.5h,3h,4h,6h, three were fed Galanga Tables, three were fed Galangin, and one was blank. Blood samples were isolated by centrifuge machine , then reserved blood serum for using.③All samples were dealed with ethyl acetate liquid-liquid extracts for three times, then keep the upper liquid, and put them in 80℃water to steam does, recover salvation before analysing with HPLC. The chromatographic condition is the same with finger printing, the detection wavelength is 267nm.Result:1. Galangin was detected in all samples from two diffrient teams, but the contents detected in blood serum were so little that only at one time point it appears highlight. But in utine and dejecta we detected more Galangin. This probably means Galangin can be absorped into blood through the Gastrointestinal path, but it might be transferred quickly. 2. The asorption And excretion of two groups were different:Cmax time point of the table extraction group is 2.5h, and other group is 1.5h;additionly,the Galangin content percent of tablet extraction group between 32. 7and 55.0% , the percent of aother group ranges 3. 6-31.8%. This just explained that single component makes different action with the whole herb in body.Conclusions: The Chinese herb is made of so many components that to get the effective mechanism and quality standards better,we must research both the tablets' components and their essential changes in vivo.