The Expression Of CD46 And CD59 In Human Colorectal Carcinoma And Paracancerous Tissues,Chronic Inflammation Of Colorectal Mucosa And The Research Of Its Clinical Significance

Objective: To detect the expression of CD46 (membrane cofactor protein,MCP) and CD59 (protectin) in human colorectal carcinoma group and control group and study the intensity differences of CD46 and CD59 in closely related clinicopathologic factors as well as investigate the significance for the intensity differences of colorectal carcinoma, a possible contribution to tumor immunity of colorectal carcinoma and the significance in clinical research.Methods: The patients with colorectal carcinoma, paracancerous tissue and chronic inflammation of colorectal mucosa were proved pathologically at the department of pathology, the first affiliated hospital of Guangxi Medical University from March 2006 to September 2006, who selected randomly were enrolled and divided into two groups. Colorectal carcinoma group included 53 cases. Control group included 52 cases( paracancerous tissue group contained 32 cases and chronic inflammation of colorectal mucosa group contained 20 cases ). The immunohistochemical staining method(ultro-sensitive-SP) was chosen to detect the expression and distribution of CD46 and CD59 in both groups. The clinical data was consulted and integrated. Nonparametric tests and related analysis of SPSS 13.0 were used.Results: 1. The positive rate of CD46 and CD59 was 92.45%, 50.00%, 35.00% and 86.79%, 59.38%, 40.00% respectively in colorectal carcinoma,paracancerous tissues and chronic inflammation of colorectal mucosa. The expression of CD46 and CD59 in human colorectal carcinoma group and control group both had notablely statistical differences, p < 0.01. Neither the positive intensity of CD46 and CD59 in paracancerous tissues group nor chronic inflammation of colorectal mucosa group had statistical differences, p > 0.05.2.The positive intensity of CD46 and CD59 in colorectal carcinoma was compared among clinicopathologic factors. For the positive intensity of CD46 in colorectal carcinoma, statistical differences existed among lymph node metastasis group, histological differentiation group and Dukes' stage group, p < 0.05. For the positive intensity of CD59 in colorectal carcinoma, there weren't statistical differences among the clinicopathologic factors, p > 0.05.3.The correlation was done between the positive intensity of CD46 and CD59 and clinicopathologic factors .The result showed that there was negative correlation between lymph node metastasis, histological differentiation and Dukes' stage respectively and the positive intensity of CD46 (r value -0.398**, -0.403** and -0.342*, p < 0.05). There was no correlation between the positive intensity of CD59 and clinicopathologic factors, p > 0.05. No association was between the positive intensity of CD46 and the positive intensity of CD59,p > 0.05.Conclusion: 1. The expression of CD46 and CD59 is respectably stronger in colorectal carcinoma than in paracancerous tissues and chronic inflammation of colorectal mucosa, which suggests a possible mechanisms of colorectal carcinoma escaping immunity attack and tumor immune tolerance.2.The positive intensity of CD46 is stronger in no lymph node metastasis group than in lymph node metastasis group, and is stronger in Dukes' A,B stage group than in Dukes' C,D stage group. The result suggests the intense staining of CD46 is playing a primary role in tumor genesis and earlier period of advancement. There is negative correlation between histological differentiation and the positive intensity of CD46 in bivariate correlate. The finding suggests that the intense staining of CD46 could be extremely important to tumor advancement,proliferation,differentiation and transfer process. Moreover it could be a high risk factor for postoperative recurrence and metastasis of colorectal carcinoma.3.There is no correlation between clinicopathologic factors and the positive intensity of CD59. No association was between the positive intensity of CD46 and the positive intensity of CD59 in colorectal carcinoma.4.CRPs could be important molecules for early diagnosis of tumor, criterion of tumor prognosis and molecule targeted therapy.