The Adriamycin (ADR) -induced Cardiotoxicity Disorder Of Intracellular Calcium Cycling And The Potential Protect Effects Of Rb1

Adriamycin (ADR) is an anthracycline antibiotic, it is one of the most effective and important anticancer agents widely used in the treatment of a number of solid tumor. However, cardiotoxicity greatly compromises the clinical application of ADR. Rb1, ginseng panaxadiol saponin monomer could increase neural plasticity in efficacy and structure; can increase proliferation and differentiation of neural progenitor cells in dentate gyrus of hippocampus of normal adult mice and global ischemia model in gerbils. Our research was aimed at the ADR-induced cardiotoxicity disorder of intracellular calcium cycling and the potential protect effects of Rb1The effects of adriamycin on the cardiotoxicity function of adult male Wistar rats were measured by the changes in intracellular Ca²⁺ concentration treatment with fluorescence. Intracellular calcium concentration ([Ca²⁺ ]i), measured by changes in fura-2 levels in response to fluorescence was significantly increased in adriamycin-treated cell with control cells. In the absence of extracellular calcium, [Ca²⁺ ]i didn't have change in adriamycin-treated (3μM)cells compared with control. Suggesting an important role of the L-type Ca²⁺ channel on membranous in the effects of adriamycin. Adriamycin-induced cardiotoxicity has been suggested to be caused by abnormal intracellular Ca²⁺ homeostasis in the myocardium, which is regulated by Ca²⁺ handling proteins such as sarcoplasmic reticulum (SR) Ca²⁺ -ATPase (SERCA), sarcolemmal Na+/Ca²⁺ exchanger (NCX) and L-type Ca²⁺ channel protein etc. However, the responsible molecular mechanisms are unclear. The present study aimed to investigate expression of mRNAs coding the proteins related to [Ca²⁺ ]i metabolism in the Adriamycin -induced cardiotoxicity of rat heart.We observed the effect of different concentrations of ADR on rat heart myocardial mechanical indexes and the concentration of Ca²⁺ in cardiac muscle in the world of the first time. Experiment results showed that the ADR has certain toxicity on heart, that can cause cardiac systolic dysfunction. This effect of ADR may has relationship with the abnormal Ca²⁺ circulation in the cell.Rb1 as an efficiency substance can antagonize the cardiotoxicity cause of Adriamycin.