The Impact Factors Of Peripheral Blood Stem Cell Mobilization Of Normal Donors

Allogeneic-Peripheral blood stem cells transplantation has a rapid development since Kessiger et. did as the beginners in 1989. It's now became the most effectual treatment in both hematological system malignant disease and solid tumor. It mainly has some thing to do with the superiorities of Peripheral blood stem cells transplantation(PBSCT) which is more simply and safety compared with bone marrow transplantation ,and PBSCT can make the re-establishment of haematogenesis of the patients much faster after transplantation.Scholars all over the world did many scientific research work on peripheral blood stem cells these years, and mostly considered that PBSCs come from bone marrow. In normal physiological estate of human body there is a dynamic balance between peripheral blood stem cell pool and bone marrow stem cell pool.PBSCs are very much lower than bone marrow stem cell(BMSCs) in normal condition. It is very important to mobilize sufficient haemopoietic stem cells(HSC) with high quality ,because it's one of the key points to promote the re-establishment of haematogenesis and the recovery of the immune system of the recipients.The most popularly used mobilizations are chemotherapy drugs , haemopoietic stem cell colony stimulating factors(CSF) and combined using of both. We can only use CSF in mobilizing healthy normal donors abide by ethnics rules. PBSCT include Autologous Peripheral blood stem cells transplantation(Auto-PBSCT), Syngeneic Peripheral blood stem cells transplantation(Syn-PBSCT) and Allogeneic Peripheral blood stem cells transplantation(Allo-PBSCT). Auto-PBSCT has more restrictions on the types of the disease and the condition of the patients, more and more patients are making choice of Allo-PBSCT. Meanwhile, family planning is a long-term policy in our country , brethren can not be easily available, irrelative donors be made as the main resource of HSC. The focus we all concern about is to get enough HSCs by limited harvestings.This experiment analysised Peripheral blood stem cells(PBSCs) yield in 251 normal donors in ten years till now in our center (the HSCT Center of the 307th Hospital of PLA) with CSF, impact of age, sex, type of CSF and the harvesting time.According to our experiment, we found that there is a significant difference between the CD34~+ cells yield in male and female donors ,and male donors seems like had better mobilization effect. Age is also has a significant correlation with the CD34~+ cells yield. Younger donors achieved better results compared with older ones .Both the impact factors sex and age have statistical meanings. There is two mobilizations have been used, Granulocyte colony-stimulating factor(G-CSF) and Granulocyte-macrophage colony-stimulating factor(GM-CSF) , use G-CSF alone can make the harvest of mononuclear cells(MNC) increased rapidly, use G-CSF combined with GM-CSF(the dose of both is equal, and the total dose of the two types of CSF is equally to the dose of single G-CSF used ), the CD34~+ cells yield is obviously increased. The first time harvest on the 5th day of mobilization, both MNC and CD34~+ cells were yield more than that began on the 4th day, and the CD34~+ cells yield had significant difference between both plan. A interesting phenomena has been noticed by us during the experiment that the peak value of CD34~+ cells is different between male and female donors. The peak value of CD34~+ cells of female donors appeared later than the male donors, we suppose it's might be matter with the estrogen or might be the cooperativity of colony stimulating factor and hormone. The actual reasons of these needs to be approved by more clinical cases and accessorial experiments in animals and ex vivo.