Experiment Study On Relationship Between In Vitro Invasion And Ability Of Intraluminal Implantation Associated With Bladder Carcinoma

Objective: To establish the high in vitro invasive subseries cell lines of three bladder neoplasm strains of BIU-87, EJ and T24, and to observe the invasive process in vitro, then to study the relationship of intraluminal implantation metastasis with the in vitro invasivenes by intravesical instillation.Methods: By using a specially constructed invasive chamber, the cells that have penetrated the matrigel and polycarbonate adipose membrane were selected and cultured to establish offspring cell lines with high invasive ability. We also observe the process of invasion in vitro by scanning electron microscope, and the growth curve, cell cycle and invasive ability of the perfusion have been compared with those of their original cells of three bladder neoplasm strains. The relationship of intraluminal implantation metastasis with the in vitro invasiveness was observed by instilling the subseries cells and their original cells in the bladder of athymic mouse.Results: The subseries cells of high in vitro invasiveness of BIU-87, EJ and T24 were accepted. After over 15 times of generation, the morphous of the subseries cells was similar with that of their original cell lines. The in vitro propagation speed s of BIU-87, EJ and T24 were elevated significantly (P<0.05). The propagation speed of BIU-87 was faster than EJ and T24 (P<0.05), and the propagation speed of EJ and T24 were no significant different (P>0.05). The proliferation index of the three cells lines were no significant different (P=0.568). The in vitro invasivenes of BIU-87 was higher than EJ and T24 (P<0.05), but the numbers of the cells that who thrued Matrigel and polycarbonate membrane dropped into downstairs of Millicell chamber were fewer than EJ and T24 (P=0.004). The in vitro invasivenes was elevated significantly (P<0.001), and the propagation speed was obviously accelerated compared with their origins (P<0.05). We observed the cells that were ellipse, comparatively large, have microvillus on it superficies and were growthing on the Matigel by scanning electron microscope. We also observe the process of cell's invasion in vitro. The in vivo experiment show that if the cells have the higher invasiveness, the higher incidence rate of implantation metastasis in the athymic mouse.Conclusion: The method of constructing subseries cell lines of bladder neoplasm strains is credible. The subseries cell lines have higher in vitro invasivenes than their origins and can be used as cell models for studies of bladder neoplasm's invasiveness and metastasis. The bladder tumor cells have the higher invasiveness, the higher incidence rate of implantation metastasis in the athymic mouse.