Protection Of BDNF Gene Transfection On The Guinea Pig Cochlea Treated With Gentamicin

Objective: To explore the protective effect of the cationic lipid-mediated brain-derived neurotrophic factor (BDNF) gene transfection of deafness on the guinea pigs treated with gentamicin.Method: The liposome -plasmid complex (pIRES2-EGFP- BDNF) was injected into the guinea pig cochlea , then the guinea pigs were treated with gentamicin (150 mg/ kg , 10 d). The DPOAE and ABR were measured at 2d after stop the genetamicin and the expression of BDNF was observed respectively ,and the hair cells were observed.Results: Compared with the group of BDNF , the DPOAE of the guinea pigs transfected with blank vector and PBS were evidently dropped ( P < 0. 05). The ABR thresholds were similar with the results of DPOAE( P > 0. 05). The hair cells with Silver nitrate stain showed that the absence of hair cells in the groups of BDNF were little severity than the compared groups. hair cell count Missing of BDNF group better than the compared groups. BDNF group and blank vector group in the fluorescence results of this study were found no significant difference, No fluorescence from the lymph fluid (PBS) group and control group that did not have fluorescent protein under a fluorescent microscope image, no color.Conclusion: a certain degree of protection of BDNF gene transfection on the guinea pig cochlea treated with gentamicin, but also for BDNF in the ears of drug toxicity on the role of providing further basis.