A Preliminary Report Of Expression Of Hepcidin Gene In Wistar Rats ACD Model Is Affected By EPO

Objective 1 To establish a rat model anemia of chronic disease (ACD) and lay a foundation for further studying pathogenesis of ACD;2 To explore the effect of hepcidin on the pathogenesis and regulation of iron metabolism of ACD;To provide experimental evidence for prevetion and treatment of ACD;3 To investigate the expression changes of the liver hepcidin gene in the formation process of wistar male rats ACD model by EPO.Methods 1 According to the different interventions,twenty-four male rats were randomly divided into three groups:group N (normal group,n=8),group M (ACD model group,n=8),group L (ACD+EPO group,n=8).The tranditional animal model of rheumatoid arthritic(RA) was eatablished by injecting Freund's complete adjuvant into rats only time.On the basis of the model,we injected Freund's complete adjuvant third again so that anemia was induced; Detection of blood,Pigmented cells are being anemia in the 60th days.That the rat model of ACD is established.2 The different groups were treated with EPO or NS;injected 2000IU/kg everyday for three days.Execute the rats after 24 hours.3 Observe the swelling extent change of the toe joints and their general state of rats;Acquisition of fresh blood and liver tissue, detection blood,liver hepcidin mRNA expression level.We observation the relationship of difference expression between hepcidin gene and anemia in the different groups.Results Anemia was induced in the rat model.In the ACD group,hepcidin mRNA expression levels increased;After intraperitoneal injection of EPO,hepcidin mRNA expression levels decreased,anemia is improved.Compared with the control group significantly (P<0.01).Conclusion 1 Different degree adjuvant anemia was induced by injecting the Freund's Complete into adjuvant arthritis rats.2 in the ACD group hepcidin mRNA expression levels in the liver increased,Maybe hepcidin plays an important role in pathogenesis of ACD.3 EPO not only could decrease hepcidin mRNA expression levels in wistar rats ACD model but also improve anemia.4 It is a good way to prevent the development of anemia ang decrease hepcidin level as early as possible,It will provide a new way for the treatment of ACD.