Pretreatment With Intragastric Administration Ultraviolet B Irradiated Donor Alloantigen Promotes Donor-Specific Tolerance To Rat Nerve Allografts

Objective:.To determine if a single intragastric administration of ultraviolet B-irradiated(UVB) donor splenocytes can prevent nerve allograft rejection and confer donor-specific immunotolerance to rat nerve allograft segments.METHODS:â‘ In the first control group,the SD rats received a syngenic SD nerve isograft, and this group named Groupâ… .â‘¡In the second control group,the SD rats received a nerve allograft from Wistar rats without pretreatment,and this group named Groupâ…¡.â‘¢In the oral-tolerance treated group,SD recipients were inoculated with UVB irradiated(1200 mj/cm2) donor splenocyte cells by intestinal tract administration 7 days before transplantation,and this group named Groupâ…¢.Alloantigen-specific tolerance has been achieved with development of a state of donor-specific T lymphocyte unresponsiveness.Using a single intestinal tract administration before transplantation,we have induced alloantigen-specific immunosuppression, which prevents rejection to allow nerve allograft regeneration,functional motor recovery and avoid the injure by second operation.Results:Pretreatment with UVB-irradiated donor splenocytes 7 days before transplantation prevented nerve allograft rejection.Pretreated animals receiving a nerve allograft recovered limb function and demonstrated morphological,histological,and electrophysiologic parameters of nerve regenteration similar to that measured in rats receiving a nerve isograft.In vitro immunological assessment by CDCD8~+ T lymphocytes,CD4~+T lymphocytes and the CD4/CD8 demonstrated a marked donor specific suppression in allografted animals pretreated with intraportal UVB-irradiated donor splenocytes.These assessments correlated with indefinite acceptance of donor nerve allografts.CONCLUSION:Gut associated lymphoid tissue is one of the significant peripheral immune organ.Antigen via intragastric will be treated and presented by antigen presenting cell,induced the immunosuppression.Oral tolerance is one of the most important way for tolerance. Pretreatment with intragastric administration promotes donor-specific tolerance.The notion of ultraviolet B irradiated donor antigen induced the donor-specific tolerance introduced in 1983. Therefore,a single pretreatment with a single intragastric dose of UVB-modified donor antigen specifically induces tolerance to peripheral nerve allografts in rats.