Antiproliferative Effect On Human Hepatocellular Carcinoma Cells By Bufalin And Its Related Mechanism

Objective To study the antiproliferative effects of bufalin on hepatocellular carcinoma cell line HepG2 and the relative mechanisms. Methods To study the inhibitive effects of bufalin on the proliferation of HepG2 cells by MTT assay, apoptosis and cell cycle distribution by flow FCM. Western blotting is used to detected the Expression of protein related to cell cycle and apotosis and the activity of survival signal transduction pathway such as PKB/AKT and JNKMAPK.Results (1)Bufalin could inhibit the growth of HepG2 cell in a concentration and time dependent manner in vitro. (2) Bufalin could cause G2/M cell cycle arrest in HepG2 cells through downregulating the leval of CyclinB1 and inactivating CDK1.(3) Bufalin could induce remarkable apoptosis in HepG2 cells, the apoptosis effect was partly caused by the up-regulated leval of mRNA and protein of Bax accompanied with down-regulated leval of mRNA and protein of Bcl-2.(4) The above cell cycle arrest and apoptosis was related to the activation of JNK MAPK signal pathway and inhibition of PKB/AKT signalpathway.