Influence Of HSP90 Inhibitor Geldanamycin On Proliferation,Invasion And B-RAF Expression Of SGC-7901 Gastric Cancer Cell

Objective To study the Infulence of HSP90 inhibitor geldanamycin on proliferation,invasion and B-RAF expression of SGC-7901 gstric cancer cell and invesgate the role of HSP90 on proliferation,invasion and apoptosis of gastric cancer and related machanisms.Methods 1. Cultures of SGC-7901 gastric cancer cell were exposured to geldanamycin ( GA) at the different concentrations of 0.20μmol/L,0.40μmol/L,0.80μmol/L,1.60μmol/L and 3.20μmol/L respectively. The morphologic change of SGC-7901 was observed under invertd microscope. Growth activity of SGC-7901 was measured by cell counting and methyl thiazoly tetrazolum ( MTT)test.The effect of GA on apoptosis inducement of SGC-7901 was derminated with Tunnel staining.2. The influenc of GA on invasion and migration of SGC-7901 was derminated with Tranwell Chamber.3.Reverse transcriptase polymerase chain reaction ( RT-PCR ) was performed to determine the expression of SGC-7901 under the influence of GA.Rusults 1. After treating with GA,the morghologic changes such as alveolation,distortion,pyknosis and detatchment were observed.The proliferation of SGC-7901 was inhibited significantly by GA in a time and cincentration depentdent way approximately. The apoptosis was induced obviously by GA of 1.20μmol/L.2.The invasion and migration of SGC-7901 were inhibited obviously with the treatment of GA of 0.60μmol/L after 48 hours( P<0.01vs control) .3.The expression of B-RAF of SGC-7901 was downregulated obviously with the treatment of GA of 1.20μmol/L after 48 hours(P<0.01vs control).Conclusions 1. The proliferation of SGC-7901 could be inhibited significantly by GA in a time and cincentration depentdent way in vitro. The apoptosis of SGC-7901 could be induced obviously by GA in vitro.2 The invasion and migration of SGC-7901 could be inhibited obviously by GA in vitro.3. The expression of B-RAF of SGC-7901 could be downregulated obviously by GA in vitro.