Promoting The Regeneration Of Rat Hepatocytes With Heparin Binding Epidermal Growth Factor-like Growth Factor After Partial Orthotopic Liver Transplantation

Objective To evaluate the effects of Heparin Binding Epidermal Growth Factor-like Growth Factor on liver regeneration after partial orthotopic liver transplantation.Methods The partial orthotopic liver transplanted model was established in SD rats with two-cuff technique.In experimental group, immediate after operation,500μg/kg HB-EGF was administered intravenously twice a day.Same vehicle of saline was administered as control.The rats were killed randomly on the 6 hour,days 2,4 and 7 after operation.The serum levels of albumin and alanine amino transferase were detected.Each liver was removed and weighed.The expression of Ki-67 anti-gen was detected by using immunohistochemistry assay.The regeneration response of hepatocytes was evaluated by flow cytometry.Results The wet weigh in HB-EGF group was significantly higher than that in control group 6 hour,2,4days after transplantation.The serum level of alanine amino transferase was lower and that of albumin was higher in HB-EGF group than in control group 6 hour,2,4,7 days after operation.The proliferating index and Ki-67 labeling index of graft in rHB-EGF group were higher than in control group 2,4days after transplantation.Conclusion HB-EGF can promote the regeneration of rat hepatocytes after partial liver transplantation.