The Expression And Significance Of NM23-H1 And IGF-â…¡ In Colorectal Cancer

OBJECTIVE: To investigate the expression of NM23-H1 and IGF-II in human colorectal carcinoma tissue, detect the relationship between them and clinical characteristics and analyze the relevance between IGF-II and NM23-H1.METHODS: NM23-H1 and IGF-II expression were examined by immunohistochemistry (SP-method) in normal colorectal mucosa,paratumoral tissues and colorectal cancer tissue. The relationship between NM23-H1 and IGF-II expression and clinicopathological factors was statistically analyzed. The relevance of IGF- II and NM23-H1 was also observed.RESULTS:1. In colorectal cancer tissue, the positive expression rate of NM23-H1 was 44.8%; in paratumoral tissues, the positive expression rate was 79.3%, in normal colorectal mucosa tissues, the positive expression rate was 90%.The expression of NM23-H1 in cancer tissues was significantly lower than that in the paratumoral tissues and normal colorectal mucosa tissues (p <0.01).2. In colorectal cancer tissues, the positive expression rate of IGF-II was 63.8%; in paratumoral tissues, the positive expression rate was 27.6%, In normal colon mucosa tissues, the positive expression rate was 20%. IGF- II protein in cancer tissue's expression was significantly higher than that in the paratumoral tissues and in normal colorectal mucosa tissues (P <0.01).3. The expression of NM23-H1 in colorectal cancer tissues have no relationship with the age, gender, and tumor size and the degree of tumor differentiation. Decrease or loss of NM23-H1 expression was significantly correlated with Dukes staging of tumors (p=0.011) and lymph nodes metastasis (p=0.011).4. The expression of IGF- II in colorectal cancer tissues have no relationship with the age, gender, tumor size and the degree of tumor differentiation. High IGF- II expression was significantly associated with Dukes staging of tumors (p = 0.009) and lymph nodes metastasis (p=0.009).5. In colorectal cancer tissues, the negative correlation existed between NM23-H1 and IGF- II expression (r=-0.547,p <0.001).CONCLUSIONS:1. In colorectal cancer tissues NM23-H1 expression is lower significantly than expression in normal intestinal mucosa tissues and paratumoral intestinal mucosa tissues. NM23-H1 expression was significantly associated with tumor Dukes staging and lymph nodes metastasis, not correlated with age, gender, tumor size and the degree of tumor differentiation.2. In colorectal cancer tissues IGF- II expression is higher significantly than expression in normal intestinal mucosa tissues and paratumoral intestinal mucosa tissues. IGF- II expression was significantly associated with tumor Dukes staging and lymph nodes metastasis , not correlated with the age, gender and tumor size and degree of tumor differentiation.3. In colorectal cancer tissues, IGF- II and NM23-H1 expression was negatively correlated.