The Research Of Comparative Proteomics Between High Malignant Bladder Transitional Cell Carcinoma And Corresponding Normal Epithelium

ObjectiveBladder cancer remains to be a focus in cancer research as it is the most prevalent type of cancer in the urinary tract. Every year there are about 330000 new carcinoma of bladder patients In all of the world. The two-tie grading system provided the new molecule base for the study of Bladder cancer. Further study may elucidate the molecule mechanism of Bladder carcinogenesis and provide potential clinical diagnositic and therapeutic targets, and have important significance. Now the pathological staging and grading is still the main means to guide the therapy and evaluate the therapy methods and therapeutic effect. However, it is not possible to predict the recurrence and understand bladder carcinogenesis by pathological classification. Therefore it is necessary to identify biomarkers that are useful in prognosis, progression, and clinical medicine.Methods1. The proteins of the aggressive bladder transitional cell carcinomas and normal tissue was separated by hand microdissection combining two-dimensional electrophoresis and Coomassie brilliant blue techniques; then the matched differentially expressed proteins of the bladder transitional cell carcinomas and normal tissue were chosen by image analysis software, whose relative value exceeded three.2. The differentially expressed proteins were identified by Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF/TOF-MS) and the function of proteins were searched through internet.Results1. The good reproducible results of 20 differentially expressed proteins were acquired; and 11 proteins were up-regulated in the bladder transitional cell carcinomas, the other 9 proteins were down-regulated.2. The peptide mass spectrometry maps and amino acid sequences were obtained successfully by the MALDI-TOF/TOF-MS technique. And the 11 proteins were identified through the database search. The 5 proteins were reported which perhaps related with the bladder transitional cell carcinomas including heat shock 60, keratin7, keratin8, annexin5, prohibitin. There were little reports about the relationship between other 6 proteins including PDIA6,14-3-3εprotein, Desmin, Tubulin beta-III, Mortalin and Annexin A4 with the bladder transitional cell carcinomas by now yet.ConclusionThe differentially expressed proteins of the bladder transitional cell carcinomas were acquired by hand microdissection combining 2-DE and MALDI-TOFTOF-MS techniques. Further study on these differentially expressed proteins need to be verificated in the gene and protein level, then it may elucidate the molecule mechanism of bladder carcinogenesis and provid potential clinical diagnositic and therapeutic targets.