The Effect Of Propofolon On C-fos Gene Of Hippocampus After Contusion And Laceration Of Brain In Rats

Objective: The acute contusion and laceration of brain patient very to see in the neurosurgery department ,The primary contusion and laceration of brain need not the surgery to treat generally But when has the secondary to harm causes acute intracranial hypertension even brain hernia forms, then has necessity of the surgery,In the technique patient's management as well as the choice have the brain protective function narcotic to be essential . As clinical new narcotic,propofolcan cut the brain metabolism rate, reduces the brain blood stream quantity, reduces the cerebrospinal fluid pressure to have the protective function to the neuron to become the research gradually the hot spot. The C-fos protein is one kind of namely early gene.it is relates the cell biochemistry change and the cell has specificity response intermediary finally to the stimulation, May takes its expression and the expression intensity the judgment nerve cell extent of damage the molecular biology symbol. The C-fos gene's high expression and the apoptosis degree is proportional. Therefore, in the technique applies has the brain protective function anaesthesia medicine, reduces the c-fos protein effectively expression quantity, after the technique patient's restoration as well as cuts the mortality rate to have the positive significance. At present after contusion and laceration of brain c-fos the protein expression influence research are few using the propofol.This test observed that the effect of propofolon on c-fos gene of hippocampus after contusion and laceration of brain in rats. after contusion and laceration of brain the c-fos protein expression conducts the research from the protein level .Methods:30 adult wistar rats,weight(241.6±10.5)g,were randomly divided into three groups:sham opreation group(A) contusion and laceration of brain contral group(B) after contusion and laceration of brain propofol group(C) which was pace infused propofol (60mg/kg). On contusion and laceration of brain in rats model .After contusion and laceration of brain was 120 minutes,cut brain and take tissue of brain. the whole brain was paraffined—inbeded and sliced.C—fos gene immunohistochemical technique was employed to observe and count on the Fos like immunoreactive (FLI) cell ,density of masccline cells,gray scale value c-fos gene of hippocampus expression was detected by immunohistoche - mistr .result: after contusion and laceration of brain, c-fos gene expression was found in the cortex . hippocampus and edge of hippocampus.expression of edge of hippocampus.was raise up obviously .And contusion and laceration of brain contral group(B)is most obvious.Only have few masccline proteinum in sham opreation group(A) .Propofol of correlate dose can abaissement expression of c-fos proteinum on each encephalic region obviously.and edge of hippocampus is the most predominance.Result:contusion and laceration of brain, the cerebral cortex, edge of hippocampus t, and brain areas and so on seahorse fringe area have the c-fos masculine protein expression, Periphery edge of hippocampus organization expresses the level obvious mark up, contusion and laceration of brain control group by the brain to express obviously. The sham-operation group only has the few masculine protein expression. The related dosage's propofol may obviously suppress the c-fos protein in various brains area expression, is especially most remarkable by the edge of hippocampus. The propofol uniform speed pump enters the group to be possible to reduce the masculine cell quantity, masculine cell density. obviouslyConclusion: contusion and laceration of brain can induce the expression of c-fos obviously,and the brain protection of propofol that can down regulation the c-fos gene.