| Cholelithiasis is a familiar disease, but it's morbidit mechanism is still not distinctive by now and needs farther study. With the development of people life level, lipid metabolism was disorganized, the incidence of cholelithiasis has been increasing every year. Now, Reverse cholesterol transport (RCT)is a question for discussion, this study is to investigate the function of the lipid absorbability and transport of gallbladder wall and the pathogenesis of cholesterol gallstone by the expression and distribution of ATP binding cassette transport A1( ABCA1) and CD68 in gallbladder wall .Objective1. To observe the expression and distribution of ATP binding cassette transport A1( ABCA1) in different gallbladder specimens , investigate their relationship with the lipid transport of gallbladder wall.2. To observe the distribution, morphology ,density and array rule of CD68 positive macrophages in different gallbladder specimens, analyse its function in the lipid transport of gallbladder wall.3. To compare betweeen the expression level of ABCA1 in the patients with cholesterol gallstone and that in controls, analyse its relationship with the formation of cholesterol gallstone.4. To compare betweeen the expression level of CD68 in the patients with cholesterol gallstone and that in controls, analyse its relationship with the formation of cholesterol gallstone.5. To compare betweeen the expression level of ABCA1 and CD68 in the patients with cholesterol gallstone and that in controls, analyse their relationship in the lipid transport of gallbladder wall, study the pathogenesis of cholesterol gallstone. .Methods1. Samples collecting gallbladder specimens after operation. 1.1 Experimental groups:the patients with cholesterol gallstone(34 cases); chronic cholecystitis without gallstone and intrahepatolithiasis (11 cases); cholesterol polypus(8 cases); acute cholecystitis(6 cases).1.2 Control group:the specimens without cholesterol gallstone (15 cases),their pathology diagnosis were almost normal .1.3 All cases were acquired pathology diagnosis by HE staining,without otheir disease and exceptional liver function.2. MethodsOlefin sections were dewaxed and dehydrated, detect the expression of ABCA1 and CD68 protein in experimental groups and control group by PV-9000 two-step immunohistochemical method, the working dilution of ABCA1,CD68 mouse monoclonal antibody is 1:200, PBS is negative control instead of first antibody.3. statistics analyse Half quantitative analysis and integral test methods; Chi-square test .Results1. The expression and distribution of ABCA11.1 ABCA1 was expressed in the apical domain of gallbladder epithelial cells,other domain was not found.1.2 The expression of ABCA1 in gallstone patients was signigicantly decreased as compared with that in controls(P <0.005).1.3 ABCA1 expression in the cholesterol polypus group and acute cholecystitis group was decreased as compared with that in controls ,with statistic significance(P <0.05).1.4 ABCA1 expression in the chronic cholecystitis without gallstone and intrahepatolithiasis group was increased, without statistic significance.2. The expression and distribution of CD682.1 CD68 was mostly expressed in the apical domain of gallbladder epithelial cells,other domains have staining also, the distribution, morphology ,density and array rule of macrophages have changed.2.2 The expression of CD68 in gallstone patients was better than that in the control group, with statistic significance(P <0.05).2.3 The expression of CD68 in the chronic cholecystitis without gallstone and intrahepatolithiasis group and the cholesterol polypus group were increased, with statistic significance.3. The comparability between ABCA1 and CD68 protein3.1 The expression of ABCA1 in cholesterol gallstone group and the cholesterol polypus group was decreased,while CD68 expression was increased , there were significantly difference between the two groups(P <0.05).3.2 The expression of ABCA1 and CD68 in the chronic cholecystitis without gallstone and intrahepatolithiasis group was increased, without statistic significance.4. The negative controls have no expression.Conclusions1. ABCA1 and CD68 protein was mostly expressed in the apical domain of gallbladder epithelial cells,indicating that gallbladder epithelial cells has the function to absorb and transport lipid .2. The expression of ABCA1 protein is decreased in cholesterol gallstone patients, indicating that ABCA1 may have some effects on the pathogenesis of cholesterol gallstone.3. The expression of CD68 protein is increased in cholesterol gallstone patients, indicating that CD68 was related with the lipid transport of gallbladder wall and the formation of cholesterol gallstone.4. There is negative relationship between the expression of ABCA1 and CD68 in gallbladder wall ,the decrease of ABCA1 protein function in gallbladder epitheliun results in that the level of the lipid transport of gallbladder wall is declined , so lipid deposits in gallbladder wall ,and induces macrophages increasing, contributes to the super-saturation of biliary cholesterol and the formation of cholesterol gallstone . Key words:ABCA1;CD68;Macrophage;Cholesterol;Cholelithiasis;Lipid transport;Gallbladder epitheliun;Gallbladder wall;Reverse Cholesterol transport...
|
PDF Full Text Request