Expression Of Adiponectin And Its Receptors On Endometrium Of Women With PCOS

Objectives:To investigate the relationship between expression of adiponectin as well as its receptors in endometrium and high risk of endometrial hyperplasia or even endometrial cancer in patients with polycystic ovary syndrome(PCOS).Methods:38 PCOS patients served as study group,while 40 cases of normal endometrium as control group.Serum FSH,LH,PRL,T,E₂,CHO,TG,FPG and FINS were determined by enzyme-linked immunosorbent assay(ELISA),and homeostasis model assessment of insulin resistance(HOMA-IR)were calculated.The expression of adiponectin and its two receptors in endometrium were detected by semiquantitive RT-PCR.In addition,immunohistochemical method was used to detect the expression of adiponectin receptorl and 2 in protein level.Results:(1)Endocrine and metabolic characteristics:FSH 6.81±0.61 IU/L in PCOS group were lower than 8.49±0.92IU/L in control group,P＜0.05;The amount of testorone,LH,CHO,TG,FINS were72.74±7.64ng/dl,9.09±0.89 IU/L,4.99±0.36mmol/L,2.06±0.77mmol/L,9.13±0.59mIU/L in PCOS,which were higher than that in control group with 36.61±6.20 ng/dl,4.55±0.72 IU/L,3.63±0.16mmol/L,1.13±0.15mmol/Land6.52±0.73mIU/L respectively,P＜0.05.(2) Adiponectin and its receptors were all detected in mRNA level in PCOS and control group,and both groups were dominated by AdipoR1,P＜0.05;the expression of AdipoR1 and total Adiponectin receptors were lower in PCOS group(0.91±0.1 and1.22±0.15)than in control women(1.13±0.19and1.45±0.29)with statistical significance (P＜0.05);there were no significant difference in Adiponectin and AdipoR2 expression between two groups.(3)The relationship between serumal FSH level and expression of total Adiponectin receptors was found(r=0.482,p＜0.05),so was FINS and AdipoR1 (r=-0.69,p＜0.05).(4)AdipoR1 and AdipoR2 were detected in protein level in endometrial tissue in both groups.Conclusion:(1)The expression of total Adiponectin receptors and AdipoR1 in mRNA level in endometrium were declined in PCOS group.So AdipoR1 might play an important role in the conversion of PCOS patients' endometrium from normal to hyperplasia or even cancer.(2)There was a positive correlation between the expression of total Adiponectin receptors in mRNA level and FSH,while negative correlation between AdipoR1 and FINS.(3)Proteins of both AdipoR1 and AdipoR2 were detected in PCOS and normal endometrium,and were mainly located on the membrane of glandular cell.