| In the chapter one, Ru bis(2, 2'-bipyridine) (2, 2'-bipyridine-4, 4'-dicarboxylic acid) N-hydroxysuccinimide ester (Ru(bpy)2(dcbpy)NHS) was synthesized and characterized using infrared spectroscopy, ultraviolet-visible absorption spectroscopy, fluorescence spectroscopy and electrochemiluminescence (ECL) spectroscopy. A maximum appeared at 672 nm on the ECL spectrum of Ru(bpy)2(dcbpy)NHS. In order to fabricate the magnetic beads modified with Ru(bpy)2(dcbpy)NHS, biotin-coated magnetic beads (bio-MB) or streptavidin-coated magnetic beads (SA-MB) was used. The ECL of the magnetic beads modified was investigated.In the chapter two, we developed a new ECL immunoassay method to detect cancer antigen 125 (CA125) using the magnetic beads modified with Ru(bpy)2(dcbpy)NHS. In this method, the primary antibody (Abi) was immobilized on the sinalized transparent indium-tin oxide (ITO) electrode. Then, the target antigen (Ag, CA125) was bound to Abi through the immunoreaction, followed by conjugating the biotinylated secondary antibody (Ab2) to Ag. Subsequently, the streptavidin (SA) was conjugated to the Ab2, followed by binding bio-MB to SA. Finally. Ru(bpy)2(dcbpy)NHS was labeled to the surface of the MBs. The ECL spectrum of the system fabricated using different Ag concentrations was recorded. The maximum of the intensity on the ECL spectra was used to quantify Ag. The linear range of the method was 5.00×10-9mol/L-1.00×10-7mol/L for CA 125. |
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