The Development Of A New Type Nerve Tissue Engineering Scaffold And Experimental Study On Repairing Of Peripheral Nerve Gaps

Part I:Development of a new type nerve tissue engineering scaffold and its combination with Bone marrow stromal cells.Objective To develop a new type nerve tissue engineering scaffold to repair the defect of peripheral nerve and observe the growth of Bone marrow stromal cells cultured in vitro on the scaffold.Methods The biomaterial was made of I-collagen and gelatin by freeze-drying method, the alignment regularities of microscopic channels and there course directions were observed under the scanning electronic microscope. The size of the micropores and the factor of porosity were also measured. The Bone marrow stromal cells were seeded on the collagen scaffolds and cultured for 5 days, then observe the growth of Bone marrow stromal cells cultured in the scaffolds.Resultes All the scaffolds were circular cylinder, the microscopic channels were arranged in parallel manners and the pore sizes of the channels were uniform. The Bone marrow stromal cells seeded in the scaffolds successfully. Conclusion The developed nerve tissue engineering scaffolds have good structure and biocompatibility, which can be used in the repair of the nerve injuries.Part II:Experimental study on repairing of peripheral nerve gaps through a collagen-gelatin scaffolds.Objective To develop a collagen-gelatin scaffold and to observe the effect of collagen-gelatin after the 10mm rat sciatic nerve defect was repaired by it.Methods The biomaterial was made of I-collagen and gelatin by freeze-dr- -ying method, and use it to bridge the 10mm gap of rat sciatic nerve. Twenty SD rats were randomly divided into two groups, group A was repaired by collagen-gelatin scaffolds, group B was repaired by auto-nerve transplantation. Sixteen weeks after the operation, all rats were detected by transmission electron microscope, S-100,GAP-43,β-tubulin class III,NF immunohistological staining and electrophysiological analysis to observe the effect of nerve regeneration by the matrix.Resultes All the scaffolds were circular cylinder, the microscopic channels were arranged in parallel manners, and the pore sizes of the channels were uniform. Sixteen weeks after the operation, a great quantity of regeneration nerves can be founded by transmission electron microscope and immuno- -histological staining, both the conduct velocity and Amp in group A were similar to the group B.Conclusion Collagen-gelatin scaffolds can improve nerve regeneration effectively.