| Gastric carcinoma is one of most severe malignancy that enormously threaten human health and life, the death rate occupy the second position of the malignancy death . In our country ,the gastric carcinoma fatality rate is the first fatality rate of malignancy in country side while it got the second position in city[1]. The most efficency therapy project of gastric carcinoma is surgical therapy, for pristine gastric carcinoma patients, the survival rate has arrived 90% after 5 years surgical therapy. Meanwhile in our country , when most of the patients made a definite diagnose of gastric carcinoma belong to advanced stage among the total 42.4% belong to IV stage[2]. If this patients simpley make a surgical therapy, the survival rate is 20% to 30% only, after 5 years.Furthermor, Gastric carcinoma has high recurrence rate after surgical therapy, 50% to 60% early gastric cancer patients will develop metabasis after operation within 2 years[3]. Accordingly running normative, reasonable, combined therapy for progression gastric cancer patients has a considerable significance for improve survival rate after operation. Since most of grastic carcinoma patients has a poor body constitution can not tolerance high intencity Chem, so serching for better curative effect and little adverse reaction Chem project is especially important. In recent years preoperative Chem has been paid generally attention (preoperative Chem also called neoadjuvant chemotherapy), can be used to advancetage grastic carcinoma, and efficially degrade carcinoma palindromia, prolong the patients life. The effect of chemotherapeutics for carcinoma cell is performed by inducing apoptosis and inhibiting cell proliferation. The key problem is selecting efficient Chem project. Out-of-body cell culture has been verificated many kinds of medicine physical chemistry factor can induce apoptosis and inhibit cell proliferation.. preoperative Chem efficially degrade carcinoma palindromia, prolong the patients life, but the effect of traditional Chem project inducing apoptosis and inhibiting cell proliferation is definite, so serching for better curative effect and little adverse reaction Chem medicine and Chem project become the key problem. Oxaliplatin is the third generation platinum group medicine, the activity of anticancer is strong , no cross tolerance with CCDP, joint action whit 5- FU, utility for many carcinoma, low adverse reaction and it has a perfect application prospective.Objective:To investigative the effect of different project of preoperative chem.(neoadjuvant chemotherapy) gastric carcinoma cell apoptosis and proliferating cell nuclear antigen apoptosis. To search one kind of more effective preoperative chem. project(neoadjuvant chemotherapy).Method: 60 cases of advanced gastric cancer patients were randomly divided into three groups(A,B and C), 20 cases in each group. Group A oxaliplatin +fluorouracil(Olp+5Fu+CF),group B fluorouracil+calcium folinate(5-Fu+CF),and group C of no Preoperative chemotherapy contrastive, the whole body vein injection administration , A group oaliplatin 130mg/m2, VD/2h,d1. fluorouracil 400mg/ m2,VD (quickly), 600mg/m2,VD22h,d1—d2. CF 300mg, IV, d1, d2. B group fluorouracil 400mg / m2,fast vein drop injection; 600mg/m2,VD 22h,d1—d2. CF 300mg IV d1—d2. using gastric carcinoma radical operation within one week after chemotherapy. To utilize TdT-mediated dUTP nick end labeling (TUNEL) and immunohistochemical means in detecting three group of preoperative chemotherapy . Gastric carcinoma cell apoptosis and proliferating cell nuclear antigen. With one group of no Preoperative chemotherapy patients act as comparision.Result :1 A group apoptosis rate of gastric carcinoma cell is 15±4.29℅ B group is 11.79±3.46℅. C group apoptosis rate of gastric carcinoma cell is 6.51±3.24℅. The disparity of each group all have statistical significance ( P<0.05 ).The mascμline rate of PCNA in A group is 26.04±9.02% ,in B group is 39.49±13.58%, in C group is 57.33±15.89%.2 bc1-2 proteinum express: A group is 33.78±3.78%; B group is 40.14±5.64% ;C group is57.34±8.13% .The disparity of each group all have statistical significance ( P<0.01 ). bax proteinum express: A group is 64.16±8.47%; B group is 43.27±5.35%;C group is 27.23±3.27% .The disparity of each group all have statistical significance ( P<0.01 ).Apoptosis controlling gene bcl-2 and bax relative value: A group is 52.67% , B group is 92.77%,C group is 210.58%. Conclusion: Both preoperative chemotherapy project can promote gastric carcinoma cell apoptosis and inhibit gastric carcinoma cell propagation. With Oxaliplatin chemotherapy project is proved obviously surpass tradition project in induced tumor cell apoptosis and inhibit tumor cell propagation.
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