Studies On Effection Of Bushen Herb And The Ras Protein Activity Of The Follicle Fluid

Objective: To observe the effect of Erzhitiangui(EZHTG) decoction on improving the quality of oocytes from experimental and clinical research, and further explore its functional mechanism from molecular, genetic level. Methods: Experimental study: 60 mice were randomly divided into four groups: the first group(15 treated with EZHTG decoction and HMG) , the second group(15 treated with EZHTG), the third group(15 treated with liuweidihuang decoction and HMG) and the last group(15 treated with HMG). Ovulation and splitting ovums were observed to study the effect of EZHTG decoction to quality of oocytes. And determine the Ras protein activity of oocytes by immunohistochemistry (SABC). Clinical study:60 patients of IVF were randomly divided into two groups: the experimental group(treated with EZHTG decoction combined with western medicine) and the control group(treated with Liuweidihuangkeli western medicine). Symptoms of renal asthenia before and after treatment and detected serum estradiol level on the day of injecting HCG, the ratio of mature oocytes, fertilization, cleavage and pregnancy were observed. Results: Experimental study indicated thatâ‘ the groups of EZHTG decoction combined with HMG, liuweidihuang decoction combined with HMG and alone HMG have no differences in effect of ovulation (P>0.05),but the first was better than the later two groups of embryo morphology, fecuudation rate, splitting ovums the grades rate (P<0.05).â‘¡Ras protein expression in normal oocytes is higher than the abnormal oocytes (P <0.01).â‘¢Ras protein expression in normal oocytes of EZHTG decoction combined with HMG and alone EZHTG decoction is higher than the normal oocytes of liuweidihuang decoction combined with HMG and alone HMG (P <0.05). Clinical study indicated that the experimental group was markedly better than the control group in ameliorating the symptom of renal asthenia and improving the ratio of mature oocytes,fertilization and cleavage (P<0.05). As compared with the control group, serum estradiol level of the experimental group on the day of injecting HCG is higher(P<0.05). Conclusion: EZHTG decoction can strengthen Ras protein activity of oocytes, whose function of improving quality of oocyte may relate to high expression of Ras protein in IGF-1 system. EZHTG decoction can regulate renal Qiyinliangxu symptoms, regulate hormone levels and increase the ratio of fertilization and cleavage.