The Distribution Of Cytoglobin In Neonatal Rat And The Localization Of Cytoglobin In Neonatal Rat Brain

Backgrounds and aimsHypoxic–ischemic damage, especially brain damage, can result from perinatal asphyxia and is an important cause of neonatal mortality and subsequent sequelae. The pathogenesy is so complicated, and the clinical therapeutics is always need to be argued. Neuroglobin and cytoglobin are two recent additions to the family of heme-containing respiratory proteins. They will provide us a new way to reveal the pathogenesy and clinical therapeutics of hypoxia-ischemic damage. Therefore, the aim of this study was to explore the distribution of Cygb in neonatal rat tissues and the localization of Cygb in neonatal rat brain.Methods(1)Western blot method was used to examine the expression of cygb in tissues(pancreas, heart, liver, lung, spleen, kidney, brain, colon and stomach)of neonatal rat.(2) The distribution of Cygb in tissues and the localization of Cygb in brain of the neonatal rat was examined by immunohistochemical method.Results1) Western blot result shown bands of 21kDa in various tissues.2) Cygb-positive cells were detected in central nervous system, digestive system, respiratory apparatus, metabolic active endocrine tissues and myocardial cells, muscle cells. Cygb-positive results in myocardial cells and muscle cells of mammalia were reported for the first time.3) Cygb-positive cells were widely distributed throughout the neonatal rat brain, including cerebral cortex, hippocampus, thalamus, hypothalamus, brain stem and cerebellum. Cygb-positive matters were located in either the plasm or the nuclei of neurons.Conclusion1) Cygb was widely distributed in central nervous system, digestive system, respiratory apparatus, metabolic active endocrine tissues and myocardial cells, muscle cells.2) Cygb was widely distributed throughout the neonatal rat brain, including cerebral cortex, hippocampus, thalamus, hypothalamus, brain stem and cerebellum.