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The Expression And Distribution Of Dendritic Cells In Nasal Polyps

Posted on:2009-04-16Degree:MasterType:Thesis
Country:ChinaCandidate:X S LinFull Text:PDF
GTID:2144360248454554Subject:Department of Otolaryngology Head and Neck Surgery
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1.Background and ObjectDendritic cells (DCs) are the most potent antigen presenting cells(APCs) and are able to induce primary immune responses. DCs possess the capability to capture, process and present antigen and induce immune diseases. DCs are special APCs which are able to present antigens to na?ve and quiescent T cells and consequently play an important role not only in initiation but also in the maintenance of inflammation and allergy diseases. (1)Nasal polyps is a common diseases in ENT. The pathogensis of nasal polyps is not clear and now most scholars think that the nasal polyps are the results of many complications. As we known, the pathologic immune system may play a key role in the onset and development of nasal polyps. The penetration of T lymphocytes and eosinophils(EOS),Th1/Th2 cells proportion unbalance and the cytokines of Th2 cells predominated are characteristic of the nasal polyps. DCs can present antigens to T cells and active T cells through co-stimulating molecules and cytokines. So, we presume DCs play an important role in pathogenesis of nasal polyps.But there still haven't direct study of the relationship of DCs and nasal polyps. So, our study want to investigate the expression, distribution and function of DCs in nasal polyps and study the role of DCs in the pathogenesis of nasal polyps.It give us a new idea of the pathogenesis of nasal polyps.2.MethodsThe study group consisted of 45 nasal patients and 10 patients with deviation of nasal septum requiring surgical treatment were assigned as control. Nasal polyps and normal inferior turbinates tissues specimens for this study were collected during surgical operation. And we divide the study group into two group: corticosteroids therapy group and no corticosteroids therapy group. All the cases were diagnosed by histological examination (hematoxylin and eosin staining). A All the case follow these two experiments.⑴Immunohistochemical staining was used to check the expression and distribution of the S-100 protein .⑵Double immunostaining method to check the CD1a and CD40 and images were analyzed with Axioplan 2 microscopy.3.Results⑴The expression of S-100 protein in nasal polyps is higher than in normal inferior turbinates, and the difference was distinct significant (P<0. 01);⑵The distribution areas,number and density of double-stained cells in nasal polyps were greater than in normal inferior turbinates(P<0. 01);⑶The S-100 protein and double-stained cells were mainly in the lamina propria below the mucous membrane.⑷The distribution areas,number and density of DCs in corticosteroids therapy group and no corticosteroids therapy group were either greater than in normal inferior turbinates, the difference was distinct significant (P<0. 01);and between corticosteroids therapy group and no corticosteroids therapy group, the difference was significant (P<0. 05).4.Conclusions⑴The number of DCs increased in the nasal polyps tissues ,it could conclude that DCs involved in pathogenesis of nasal polyps⑵There were some CD40 positive DCs suggest that it was related to the a reciprocity relationship of DCs and T lymphocytes. DCs induced the infiltration and differentiation of T cells by co-stimulating molecule and cytokines. Followed, DCs induced the Th1/Th2 cells proportion unbalance and the predominate of Th2 cells cytokines through the cell and serum mechanism. In addition, DCs could induced the infiltration of EOS by IL-5.⑶Corticosteroids therapy distinctly decline the number of DCs in the nasal polyps. Corticosteroids could restrain the remove and assemble of DCs,restrain the antigen-presenting and restrain the activation of T cells so that it could restrain the inflammatory and allergic immunoreaction.⑷DCs were mainly in the lamina propria below the mucous membrane,it show that the lamina propria below the mucous membrane was the place DCs interacted with the other inflammatory cells.
Keywords/Search Tags:Nasal polyps, Dendritic cells(DCs), Corticosteroids therapy
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