A Novel HPV Genotyping Method Based On Polymerase Chain Reaction And Cross-Contamination-Proof Nucleic Acid Detection Device

Objective: Develops a novel genotyping method for detecting the four types of human papillimovirus (HPV6, HPV11, HPV16, HPV18) which based on the polymerase chain reaction (PCR) and cross-contamination-proof (XCP) nucleic acid detection device.Methods and Results: After the sample preparation and PCR amplification, the XCP nucleic acid detection device was used for HPV detection and genotyping .Compared the results of 137 clinical HPV positive samples with real time PCR, the coordination rate was beyond 97 percent.Conclusion: The results showed that the new genotyping method can detect HPV genotypes and is simpler, faster, more accurate, cheaper, no expensive-equipment needed and cross-contamination-proof comparing with other method. It could be used in the small hospital and the clinics in resource-limited area and have an important promote value.