Protective Effect Of Deuterohemin His Peptide-6 (DhHP-6) On Cerebral Ischemia Reperfusion Injury In Rats

Deuterohemin-His-Peptide 6(DhHP-6) is a six-peptide-derivation included deutohaemoglobin,which based on antiscorbic acid peroxydase and minienzyme composition.It was a simulacrum of peroxydase has the activity of peroxydase and to prevent damaging of hyperoxygen and free radical.Pharmacological research demonstrated that DhHP-6 cordis muscle with meager blood,inhibitting atherosclerotic occurrence and development.It also had the potent effects on restraining the formation of caligo lentis,antiaging,enhancing organism immunity.At present,little materials has been published about the effects of DhHP-6 on cerebral ischemia reperfusion injury.The experiment rats cerebral ischemia reperfusion injury was induced by middle cerebral artery occlusion to observe the influence of SOD,MDA and LDH in serum;and the content of SOD,MDA and NO in cerebral tissue.We made ischemia reperfusion model in rats to study the effects of DhHP-6 on neurological scale,neurocyte morphology changes and infarct size to explore the mechanism,accordingly provided the theory of clinical application in cerebral ischemia.Experimental result showed that,MDA,LDH released and decreased SOD activity after cerebral ischemia reperfusion in rats.DhHP-6 (1mg/kg,0.3mg/kg,0.1mg/kg) groups significantly inhibite MDA,LDH release(P＜0.01,P＜0.05) and improve SOD activity(P＜0.01,P＜0.05) in serum;and obviously restrain NO and MDA enhance(P＜0.01,P＜0.05), striking elevate SOD activity(P＜0.01,P＜0.05) in cerebral ischemia. After reperfusion,modle rats have distinct action deficits and increase neurological scores.DhHP-6(1mg/kg,0.3mg/kg,0.1mg/kg)groups can reduced neurological scores to differently extent lighten behavior deficits (P＜0.001,P＜0.01,P＜0.05 ),modle rats have distinct action deficits.As a result of the changes of neurocytes morphology,neurons were serious edema,cytoplasma was stained slightly,nucler were contracted and stained deeply after reperfusion.DhHP-6(1mg/kg,0.3mg/kg) groups educed cellular edema,maintained the neurocyte morphology. DhHP-6(1mg/kg,0.3mg/kg) groups could significantly deflate the infarct size(P＜0.01,P＜0.05)Based on the above experiments,it has been demonstrated that DhHP-6 increased the ability of removing free radicals,decreased lipid peroxidation,protect both integrity and stability of cellular membrane, inhibited production of NO,decreased the damages of brain tissue and neurocyte then protected the neurons from cerebral ischemia reperfusion injury.