Power For Focal Cerebral Ischemia And Reperfusion In Serum Il-1¦Â, Il-8 And Brain Free Radical Research

Cerebrovascular disease is a kind of common disease in clinic attacked by higher morbidity and unfavorable prognosis. It is seriously harm for the human health due to its first disability and lethality rate among three major causes of death. Therefore, it is the one of the most important project of the clinical and experimental research to study phathophysiologic mechanisms and find the suitable treatment method for cerebral ischemia.Ischemic cerebral injury is a result of joint action of multiple phathophysiologic mechanisms with complicated procedure, in which the inflammatory response are very important process. Cerebral injury caused by ischemia and reperfusion would be abated if the levels of WBC (white blood cell) and CK (cytokine) were decreased, inflammatory response were inhibited and free radical production were reduced.The rat model of focal cerebral ischemia was created by middle cerebral artery occlusion(MCAO) in the experiment, and the levels of WBC in circulation, IL-1 P and IL-8 in serum, MDA and GSH in the brain at the different reperfusion time (Omin,6h,24h) together with the effects of electro-acupuncture selecting Baihui and Renzhong points belonged to Du meridian on them were observed. The study aimed to detect the effects of electro-acupuncture on inhibiting inflammatory cytokines and free radical in different reperfusion time after cerebral ischemia at early stage. The mechanism by which acupuncture therapy protected the cerebral injury was furthered to explore; therefore, the experimental results may be regarded as the reliable basis for guiding acupuncture clinical application.The results show as follows:(1) The level of WBC in circulation was highly decreased after cerebral ischemia-reperfusion by applying electro-acupuncture, and it induced the more significant results in cerebral ischemia early stage and ischemia-reperfusion 6h. Thus the study shows that the mechanism of acupuncture protection function on cerebral ischemia-reperfusion injury is related to the reduction of WBC level.(2) The levels of IL-1 3 and IL-8 in serum in the electro-acupuncture groups were lower than those in the model control groups, which the level of IL-1 P in serum of the electro-acupuncture group at reperfusion 6h and the level of IL-8 in serum of the electro-acupuncture group at reperfusion 24h were obviously deceased. The results suggest that not only may acupuncture function as abating the role of WBC promoting the CK release though reducing the level of WBC after cerebral ischemia, but also cause the lower levels of IL-1 P and IL-8 in serum directly then play an important role on cerebral protection.(3) Acupuncture had the function on suppressing the free radical production and lipid peroxidation and influenced on the reperfusion groups especially, therefore, the death procedures of central neural cells were delayed. There is a suggestion that acupuncture has been proved to be effective for cerebral ischemia by the action of protection on ischemia-reperfusion injury.In conclusion, our present study demonstrates that acupuncture therapy may play a significant role in protecting cerebral ischemia-reperfusion injury, which the one possible mechanism of acupuncture may be involved in abating the brain damage caused by inflammation and free radical during the process of cerebral ischemia-reperfusion. The results also show that it is important to inhibit inflammation response and regulate immune reaction for the action of acupuncture in treating acute cerebral ischemia. In essence, acupuncture therapy may bring on optimal and entire regulation function by way of the extensive adjustment to nerve neurohumor and immune system.